Stage-Specific Oligonucleotide Primers for the Diagnosis of Toxoplasmosis Among Iranian Pediatric Heart Transplant Recipients; Evaluation of Cotrimoxazole as a Preventive Therapy

AUTHORS

Mohammad Mahdavi ORCID 1 , Abdoulreza Esteghamati ORCID 2 , Khadijeh Khanaliha ORCID 2 , * , Shirin Sayyahfar 2 , Elahe Orang 1 , Farah Bokharaei-Salim 3 , Saba Garshasbi 4 , Borna Salemi 5

1 Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran

2 Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

3 Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

4 Vice Chancellor for Health, Iran University of Medical Sciences, Tehran, Iran

5 Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

How to Cite: Mahdavi M, Esteghamati A, Khanaliha K, Sayyahfar S, Orang E , et al. Stage-Specific Oligonucleotide Primers for the Diagnosis of Toxoplasmosis Among Iranian Pediatric Heart Transplant Recipients; Evaluation of Cotrimoxazole as a Preventive Therapy, Arch Pediatr Infect Dis.2021 In Press(In Press): e111940.

ARTICLE INFORMATION

Archives of Pediatric Infectious Diseases: In Press (In Press); e111940
Published Online: May 25, 2021
Article Type: Research Article
Received: December 10, 2020
Revised: February 14, 2021
Accepted: March 14, 2021
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Abstract

Background: Toxoplasmosis is an opportunistic infection that influence on solid organ transplant (SOT) recipients. The parasite transmission may be occurred from a Toxoplasma-seropositive donor to a Toxoplasma-seronegative recipient by organ transplantation. In this study, a nested PCR was carried out using different primers the B1, SAG4 and MAG1 genes to asses Toxoplasma infection in pediatric heart transplantation at Shahid Rajaei Heart Center in Tehran.

Methods: Blood samples were collected from 46 pediatric heart transplant patients between the ages of 1-17 years referred to Rajaie Cardiovascular, Medical and Research Center during 2018-2019.All pateints were on oral administration of Trimethoprim-sulfamethoxazole (cotrimoxazole) .Blood samples were collected and peripheral blood mononuclear cell (PBMC)  isolation using Ficoll gradient method was performed. DNA extracted from PBMC and nested PCR was carried out. Serologic tests were performed using ELISA to determine IgG and IgM anti - Toxoplasma gondii antibody.

Results: The results of serologic tests showed that all of 46 patients had negative anti-Toxoplasma gondii IgM antibody. Furthermore 6(13.05%) and 3 (6.5 %) out of the 46 patients were positive for IgG Toxoplasma gondii antibody before and after transplantation respectively. All of 46 pateints were evaluated using PCR with B1, MAG-1 and SAG-4 primers and PCR results were negative. 

Conclusion:  In general due to the negative results of Toxoplasma with PCR using B1, MAG-1 and SAG-4 bradyzoite genes; it is possible that the result obtained in this study is due to prophylaxis with cotrimoxazole.

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