Genotypic Correlation of a Virologic Response to Lamivudine, Stavudine and Nevirapine in Patients for Whom Combination Therapy Had Failed

AUTHORS

Mirza Khalil Bahmani 1 , Ayyoob Khosravi 1 , * , Fereidoun Mahboodi 2 , Ramin SarramiForooshani 2

1 HIV and Hepatitis Research Center, Shiraz University of Medical Sciences, Gerash, IR Iran

2 Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IR Iran

How to Cite: Bahmani M K, Khosravi A, Mahboodi F, SarramiForooshani R. Genotypic Correlation of a Virologic Response to Lamivudine, Stavudine and Nevirapine in Patients for Whom Combination Therapy Had Failed, Arch Clin Infect Dis. Online ahead of Print ; 3(4):215-9.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 3 (4); 215-9
Article Type: Brief Report
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Abstract

Background: Resistance is the consequence of mutations that emerge in the viral proteins targeted by antiretroviral agents. Thus, we focused our attention on mutations in HIV-1 reverse transcriptase to define their association with specific NRTIs and NRTI resistance mutations at therapeutic failure.

Patients and methods: The study population included 5 Iranian HIV-positive patients referring to Counseling Behavioral Modification Center in Shiraz who received a combination of antiretroviral therapy (lamivudine, stavudine and nevirapine). PBMC DNA was isolated from blood and PCR was performed to produce a 1200 bp amplicon and resolved by electrophoresis on a 0.7% agarose TBE gel, visualized with ethidium bromide. PCR products from HIV-1- infected patients were cloned into pCR2.1TOPO, then sequenced. Finally, sequence data were analyzed.

Results: Results showed drug resistance in 2 patients, of whom one had NNRTI resistance mutations (M230G, L234R and K238H) and other had both NRTI (V75M) and NNRTI (F227L) resistance mutations.

Conclusion: Confirmation of genetic resistance in HIV-positive patients who show therapy failure can help physicians to change their drug regime in order to achieve better outcome.

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