Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran


H. Yaghoubi 1 , A. Ghalyanchi Langeroudi 1 , * , V. Karimi 2 , S. A. Ghafouri 3 , M. Hashemzadeh 4 , H. Hosseini 5 , M. H. Fallah Mehrabadi 4 , F. Sadat Mousavi 1 , H. Najafi 6

1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of clinical sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

4 Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

5 Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

6 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

How to Cite: Yaghoubi H, Ghalyanchi Langeroudi A, Karimi V, Ghafouri S A, Hashemzadeh M, et al. Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran, Arch Razi Inst. 2019 ; 74(4):e100934. doi: 10.22092/ari.2018.116786.1176 .


Archives of Razi Institute: 74 (4); 349-355
Published Online: December 01, 2019
Article Type: Research Article
Received: January 11, 2020
Accepted: April 22, 2018


Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to describe the epidemiology of GCOVs in avian species. The present study was conducted to detect GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight  different bird species ((Chickens (Gallus gallus), Pheasant (Phasianus colchicus), Turkey (Meleagris gallopavo), Partridge (Perdix perdix), Quail (Coturnix coturnix), Duck (Anas platyrhynchos), Goose (Anserini),and  Guinea fowl (Numididae)) were collected, the viral RNA was extracted, the RT-PCR was performed using QIAGEN one step RT-PCR kit and the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates. Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran.  According to our results, GCOVs are circulating in different avian species, and further studies are needed to isolate these viruses and evaluate their pathogenesis.

© 2019, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.