Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran

AUTHORS

A. Hosseini-Chegeni 1 , M. Tavakoli 2 , Gh. Goudarzi 3 , Z. Telmadarraiy 4 , 5 , M. Sharifdini 6 , F. Faghihi 7 , 8 , * , M.K. Ghanbari 9

1 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran

3 Department of Medical Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran

4 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran High Education Institute of Rahyan Novin Danesh (RND), Sari, Iran

5 High Education Institute of Rahyan Novin Danesh (RND), Sari, Iran

6 Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

7 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

8 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

9 Department of National Program of Zoonotic Disease, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran

How to Cite: Hosseini-Chegeni A, Tavakoli M, Goudarzi G, Telmadarraiy Z, Sharifdini M, et al. Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran , Arch Razi Inst. 2020 ; 75(1):e103435. doi: 10.22092/ari.2019.122532.1259.

ARTICLE INFORMATION

Archives of Razi Institute: 75 (1); 39-46
Published Online: March 01, 2020
Article Type: Research Article
Received: April 06, 2020
Accepted: February 20, 2019
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Abstract

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticumHyalomma dromedariiRhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.

© 2020, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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