Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats

AUTHORS

S. Moshkelani 1 , A. Asghari 1 , * , G. Abedi 1 , A. Jahandideh 1 , P. Mortazavi 2

1 Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

How to Cite: Moshkelani S, Asghari A, Abedi G, Jahandideh A, Mortazavi P. Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats, Arch Razi Inst. 2020 ; 75(1):e103473. doi: 10.22092/ari.2018.123458.1251.

ARTICLE INFORMATION

Archives of Razi Institute: 75 (1); 83-91
Published Online: March 01, 2020
Article Type: Research Article
Received: April 06, 2020
Accepted: November 21, 2018
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Abstract

This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO4 was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO4, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO4 decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO4 (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO4 (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO4 on testicular IR injury in rats. 

© 2020, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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