Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran

AUTHORS

A. Dalimi 1 , * , F. Jameie 1 , A. Mohammadiha 1 , m. Barati 2 , S. Molaei 1

1 Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Infectious Diseases Research Center, Aja University of Medical Sciences, Tehran, Iran

How to Cite: Dalimi A, Jameie F, Mohammadiha A, Barati M, Molaei S. Molecular Detection of Hepatozoon canis in Dogs of Ardabil Province, Northwest of Iran, Arch Razi Inst. 2017 ; 72(3):e85431. doi: 10.22034/ari.2017.108389.

ARTICLE INFORMATION

Archives of Razi Institute: 72 (3); 197-201
Published Online: September 18, 2016
Article Type: Observational Study
Received: April 26, 2016
Accepted: September 18, 2016
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Abstract

Hepatozoon species are protozoan parasites that infect some animals such as birds, reptiles, amphibians, and carnivores. Previous studies performed on canine hepatozoonosis in Iran have never used molecular techniques for diagnosis of this disease. The main objective of the present study was to detect Hepatozoon canis in the blood of dogs using polymerase chain reaction (PCR) method and sequencing. A total of 104 blood samples were collected from dogs of Meshginshahr County (Ardabil Province), and DNA was extracted from blood samples by dint of DNG-plus Extraction Kit. Then, 18S rRNA gene was amplified by using the conventional PCR methods. PCR products yielded an amplicon of the approximate length of 897 bp for all the positive samples. Twenty-four out of the 104 (23.07%) samples were found to be positive for H. canis. This rate of infection is relatively high among dogs in Ardabil Province. Sequence analysis confirmed the molecular identity of 99% of the samples by comparison with GenBank profiles. This is the first report of molecular detection of H. canis from Iran.

© 2017, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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