A Molecular Study on Hepatozoon canis Infection in Dogs in Tehran (Iran)

AUTHORS

R. Soltani 1 , A. Dalimi 2 , *

1 Research Expert,Faculty of Industry and Agriculture,Department of Microbiology, Standard Research Institute (SRI),Standard squre, Karaj, Alborz

2 Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

How to Cite: Soltani R, Dalimi A. A Molecular Study on Hepatozoon canis Infection in Dogs in Tehran (Iran), Arch Razi Inst. 2018 ; 73(4):e92066. doi: 10.22092/ari.2017.110293.1125.

ARTICLE INFORMATION

Archives of Razi Institute: 73 (4); 257-263
Published Online: December 01, 2018
Article Type: Journal Article
Received: April 08, 2019
Accepted: September 27, 2017
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Abstract

Hepatozoonosis is a protozoal disease caused by various species of Hepatozoon. This parasite is transmitted from tick; the main vector of Hepatozoon canis is usually the brown dog tick (Rhipicephalus sanguineus). However, several species of ticks are disposed as the alternative vectors. Dogs are usually infected by eating the tick or a part of the tick organ infected by the mature oocysts containing infectious sporozoite. In the current study, a total of 145 blood samples were collected from the cephalic vein of pet, stray, and shelter dogs in Tehran. To conduct this study, first thin blood smears were prepared from all the samples and stained with the Giemsa method. Then, after extraction of DNA from the blood samples, in order to trace Hepatozoon canis, the 18S rRNA gene segment of the parasite was amplified using polymerase chain reaction (PCR). To confirm the PCR-positive results, five randomly selected PCR-positive samples were sequenced. According to the results, through direct observation of microscopic slides, no infection of H. canis parasite was observed, but according to the PCR results, 32 out of the 145 blood samples were found to be infected by H. canis.  In this study, infection to H. canis in older dogs was higher than in young dogs, and more male dogs were found to be infected by the parasite compared to female dogs; but no significant difference was observed in this regard (P > 0.05). Moreover, stray dogs showed a significantly higher rate of infection, compared to the pet and shelter ones (P < 0.05).

© 2018, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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Dalimi A. says:

Please change my email address from [email protected] to: [email protected] Best regards, Dalimi A.