Biochemical Responses to Level-1 Yo-Yo Intermittent Recovery Test in Young Tunisian Football Players


Omar Hammouda 1 , * , Hamdi Chtourou 5 , Anis Chaouachi 1 , Henda Chahed 2 , Nidhal Zarrouk 3 , Abdelhedi Miled 2 , Karim Chamari 4 , Nizar Souissi 4

1 Research Laboratory Sport Performance Optimisation, National Center of Medicine and Science in Sport, Tunisia

5 Research Unit (EM2S), High Institute of Sport and Physical Education, Sfax University, Tunisia

2 Laboratory of Biochemistry, CHU Farhat Hached, Sousse, Tunisia

3 Neurophysiologie de la Vigilance, de l'Attention et des Performances, Service d'Explorations Fonctionnelles du Systme Nerveux, CHU Sahloul, Sousse, Tunisia

4 High Institute of Sport and Physical Education, Ksar-Sad, Manouba University, Tunisia

How to Cite: Hammouda O, Chtourou H, Chaouachi A, Chahed H, Zarrouk N, et al. Biochemical Responses to Level-1 Yo-Yo Intermittent Recovery Test in Young Tunisian Football Players, Asian J Sports Med. Online ahead of Print ; 4(1):34522. doi: 10.5812/asjsm.34522.


Asian Journal of Sports Medicine: 4 (1); 23-28
Published Online: September 28, 2012
Article Type: Research Article
Received: March 30, 2012
Accepted: August 25, 2012


Purpose: The aim of this work was to investigate the metabolic and muscle damage responses after the level-1 Yo-Yo intermittent recovery test (YYIRT) in young football players.

Methods: Fifteen male football players (17.42 0.2 yrs, 69.91 4.4 kg, 178.64 3.8 cm; mean SD) participated in this study. Fasting blood samples for various biochemical parameters (i.e. lactate (Lac), glucose (GLC), triglycerides (Tri), creatine kinase (CK), uric acid (UA)) collected from a forearm vein after 5-min of seated rest and 3-min after the test. Moreover, rating of perceived exertion (RPE) and maximal heart rate during and after the YYIRT were recorded.

Results: Mean levels of the selected biochemical markers were raised after the YYIRT exercise (P<0.001 for the other markers). Moreover, lipid parameters increased significantly after the test (P<0.01 for Tri and P<0.001 for HDL).

Conclusion: These findings confirm the higher metabolic demand of aerobic as well as anaerobic metabolism and reflect a significant mobilization of purine cycle during the YYIRT. The increase of muscle damage markers also reflects the higher anaerobic solicitation. From these findings, we can conclude the importance of aerobic and anaerobic metabolism during soccer-specific endurance performance (i.e. YYIRT, soccer match).

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