Hepatitis B Virus DNA is Absent in Lebanese Blood Donors Who Are Positive for Both Anti-Hbc and Anti-Hbs Antibodies


Ghada Kchour 1 , Mohammad Fayyad-Kazan 2 , * , Antoine Haddad 3 , Bassam Badran 1 , Mahdi Tarhini 4 , *

1 Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, Hadath, Lebanon

2 Institut de Biologie et de Medecine Moleculaires, Universite Libre de Bruxelles, 6041 Gosselies, Belgium

3 Department of Clinical Pathology and Blood Banking, Sacre coeur Hospital, Lebanese University, Lebanon

4 Faculty of Health Sciences V, Lebanese University, and director of Clinical Pathology and Blood Banking Department , Raee Hospital, Saida , Lebanon

Corresponding Authors:

How to Cite: Kchour G, Fayyad-Kazan M, Haddad A, Badran B, Tarhini M. Hepatitis B Virus DNA is Absent in Lebanese Blood Donors Who Are Positive for Both Anti-Hbc and Anti-Hbs Antibodies, Hepat Mon. 2017 ; 17(2):e44787. doi: 10.5812/hepatmon.44787.


Hepatitis Monthly: 17 (2); e44787
Published Online: February 7, 2017
Article Type: Research Article
Received: December 14, 2016
Revised: January 11, 2017
Accepted: February 4, 2017


Background: A major risk during blood transfusion is the transmission of infectious agents such as Hepatitis B virus (HBV). Screening for anti-HBc antibodies has long been used to test HBV infection and thus, blood safety. However, the development of advanced tools enabling HBV-DNA detection rendered the diagnostic benefit of anti-HBc test uncertain.

Objectives: In this study, we aimed to evaluate the relationship between HBV-DNA, anti-HBs, and anti-HBc loads.

Methods: Sera of 7200 blood donors were first screened for HBs antigen (HBsAg) and anti-HBc antibody. Samples that were found to be HBsAg-negative but anti-HBc-positive were further tested for anti-HBs and HBV-DNA.

Results: Of the 7200 tested samples, 7143 (99.2%) were HBsAg-negative, while the remaining 57 (0.8%) samples were HBsAg-positive. Among the HBsAg-negative samples, 490 (6.8%) were anti-HBc-positive. Of the anti-HBc positive samples, 397 (81%) were anti-HBs-positive, while the remaining 93 (19%) samples were anti-HBs-negative. Interestingly, all of the anti-HBc positive samples, which also were positive for anti-HBs, exhibited negative HBV-DNA results. On the other hand, the anti-HBc positive samples, which were negative for anti-HBs, showed both negative and positive HBV-DNA results.

Conclusions: Blood samples which are positive for both anti-HBc and anti-HBs are characterized by negative HBV load.

1. Background

Hepatitis B virus (HBV) infection is a serious global public health issue and the 10th leading cause of mortality, worldwide, with more than 1 million annual deaths (1, 2). Infection with HBV can be diagnosed either after developing hepatitis-related symptoms or following routine blood testing. During the acute phase of infection, IgM antibodies against the Hepatitis B core antigen (HBcAg) predominate. As infection evolves, IgM anti-HBc declines in titer, while IgG anti-HBc can persist for many years (3, 4). Accordingly, anti-HBc is considered as a major serological marker for HBV infection (5). Positivity for both anti-HBc and HBsAg (Hepatitis B surface antigen) indicates ongoing infection (6), while antibody response against HBsAg (anti-HBs) appears only during the late phase of infection (6). Screening for serum HBsAg is widely used as a major test for HBV infection. Although HBsAg disappearance was long considered as an indication of HBV clearance and infection elimination, nowadays, it is well established that HBsAg might not be detected in the serum of even infected patients (7). This can be explained by either infection chronicity where HBsAg levels are maintained below the detection limit or relative HBsAg synthesis downregulation (8, 9). Noteworthy, some HBsAg-negative but anti-HBc-positive individuals, as in the case of OBI (occult hepatitis B infection), are characterized by continuous HBV replication (10, 11), indicating that the absence of HBsAg in blood of putatively healthy individuals could not serve as a marker for the absence of circulating HBV. Moreover, as anti-HBc can persist for many years, even after total recovery of the patient, anti-HBc screening test, being implemented in some countries such as Lebanon, in order to decrease the risk of post-blood transfusion HBV infection, might give false positive results regarding the actual state of the individual and thus, leading to high discard rate of blood units.

2. Objectives

Aiming at identifying an easy and reliable strategy that would enable the specification of blood safety, we evaluated, in this study, the prevalence of HBV-DNA in correlation with the presence of anti-HBc and anti-HBs in Lebanese blood donors. Our results identified the absence of HBV-DNA in all donors which are simultaneously positive for anti-HBc and anti-HBs antibodies. Our data indicate that screening anti-HBc positive samples for anti-HBs is highly beneficial for determining blood safety and avoiding loss of precious blood samples during blood transfusion.

3. Methods

In order to ensure the blood supply as safe as possible, all donors were screened to meet certain eligibility criteria where the donor must be at least 17 years old, with a pulse of 50 - 100 beats per minute (no abnormalities), body temperature of ≤ 37.5ºC (orally), body weight of > 50 Kg, hemoglobin level of ≥ 12.5 g/dL, hematocrit of ≥ 38%, and blood Pressure of: ≤ 180 mm Hg systolic and ≤ 100 mmHg diastolic. Moreover, infectious disease screening tests for HBsAg, anti-HBc, anti-HCV (Hepatitis C virus), HIV-1 (Human immunodeficiency virus-1), and HIV-2 antibodies as well as Venereal Disease Research Laboratory (VDRL) serological test for syphilis were carried out for each donor. The donors were also screened for disease risk factors using a health history questionnaire where they were asked specific questions regarding lifestyle, health, medical, and travel history.

During the time frame of this study (2015), 7200 blood samples from voluntary anti-HCV- and anti-HIV-negative blood donors in three different Lebanese governorates (Mount Lebanon, South, and Beirut) were evaluated for HBsAg. The number of patients participating from each of the mentioned governorates was 1320, 3400, and 2480, respectively. HBsAg-positive donors were excluded and HBsAg-negative donors were considered for inclusion in this study. Samples that were found to be anti-HBc positive were further tested for anti-HBc IgM, anti-HBs, and HBV-DNA.

3.1. Serology

A 10-ml blood sample was taken from each donor during the donation visit, centrifuged, aliquoted, and stored frozen at -70°C before being shipped to the Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, to be tested for HBV-DNA load.

Screening for HBsAg, anti-HBc, anti-HBc IgM, and anti-HBs was performed at Raee Hospital by using bioMerieux VIDAS® system ELFA (Enzyme Linked Fluorescent Assay) technology, with no carryover risks. Serum anti-HBs titer > 10 IU/L was considered positive. Relative sensitivity with VIDAS anti-HBc (Total II ref 30314) is 99.56% (95% confidence interval: 97.46% - 99.9%). Relative specificity of VIDAS HBsAg (Ultra ref 30315) is 100.00% as clinical sensitivity (95% confidence interval: 96.38 % - 100.00%). The diagnostic specificity of VIDAS anti-HBs (Total II ref 30318) is 99.0% (95% confidence interval: 98.1% - 99.5%).

The diagnostic sensitivity of VIDAS anti-HBs (Total II ref 30318) is 99.0% (95% confidence interval: 98.2% - 99.5%) and the measurement range (cut-off) is 3-500 mlU/ml. Relative sensitivity for VIDAS HBc IgM (ref 30439) is 100% (95% confidence interval: 97.98% - 100%) and the measurement range is 0-200PEIU/ml. Relative specificity for VIDAS HBc IgM II on a clinical population is 95.50% (95% confidence interval: 92.51% - 97.33%).

3.2. HBV-DNA Detection

Viral DNA extraction from plasma samples was performed using the Exiprep Dx viral DNA/RNA kit (# K-4471 from Bioneer - South Korea) on the Exiprep 16 Dx fully automated extractor of Bioneer. Then, the 5ul eluted DNA was added to the lyophilized HBV PCR mix and internal control (ref. Accu Power HBV quantitative PCR kit #HBV-1111 from Bioneer; Republic of Korea; Limit of Detection: 9.55 IU/ml (with 95% probability); Limit of Quantification: 50 IU/ml), and were then run on the Exicycler 96 real-time PCR machine of Bioneer along with 5 different standards. Calibration curve was plotted by cycler software and concentrations of unknown samples were determined. Three DNase/RNase free water negative controls, one non-template control, and five standard positive controls [SPC1, SPC2, SPC3, SPC4, and SPC5 (i.e. standards included in the kit) with the concentrations of 103, 104, 105, 106, and 107 copies/reaction, respectively) were added per run. Strict precautions were followed to avoid possible contamination. Only the data that were reproducible and showed no false positive results in the negative controls were used.

4. Results

A total of 7200 blood samples were collected from volunteer donors. 33%, 45%, and 22% of the donors were in the age range of 20 - 29, 30 - 39, and 40 - 49 years, respectively (Figure 1). 98% of the donors were male, 2% were female, 95% were first-time donors, and 5% were regular donors. The samples were screened for HBsAg and anti-HBc (Figure 2). In total, 99.2% (7143/7200) were HBsAg-negative, while the remaining 0.8% (57/7200) samples were HBsAg-positive (Figure 2). In the second step, the HBsAg-negative samples were screened for the presence of anti-HBc. The anti-HBc positivity rate was 6.8% (490/7143) (Figure 2). It is noteworthy that all the anti-HBc positive samples were negative for anti-HBc IgM (Table 1), and thus suspected to have OBIs. The anti-HBc positive samples were then screened for anti-HBs positivity/negativity as well as for the presence/absence of HBV-DNA. Among the tested samples, 81% (397/490) were positive for anti-HBs and they were all negative for HBV-DNA (Figure 2 and Table 1). On the other hand, the remaining 19% (93/490) were negative for anti-HBs, whereas HBV-DNA appeared to be positive in 2.85% (14/93) of this population (viral load range: 100 to 300 IU/ml) (Figure 2 and Table 1). In general, these observations demonstrate a direct correlation between the absence of HBV-DNA and the simultaneous presence of anti-HBc and anti-HBs antibodies.

Figure 2. Flow Chart of the Laboratory Process During This Study
Table 1. Prevalence of Anti-HBs, Anti-HBc IgM, and HBV-DNA Among 490 (HBsAg-Negative, Anti-HBc-Positive) Blood Donorsa
Anti- HBs
397 (81) positive
93 (19) negative
Anti- HBc IgM
397 (81) negative
93 (19) negative
397 (81) negative
14 (2.85) positive

aValue are expressed as N. (%).

5. Discussion

Ensuring blood safety is a global issue since blood transfusion serves as a vital therapy for managing different medical and surgical conditions where a huge number of lives can be rescued following blood administration. Despite that, blood transfusion might represent a major risk factor for the transmission of different infectious agents among which is the hepatitis B virus (HBV). Following HBV infection, antibody responses to different HBV proteins, including anti-HBc and anti-HBs, are detected in human sera (12). Following clearance of transient HBV infection, anti-HBc level remains high for longer periods than other HBV markers, thus serving as a universal indicator for HBV infection (13). The “anti-HBc” serological status could be attributed to different parameters among which are: (a) passive transfer of anti-HBc; (b) a recent HBV infection during which HBsAg has disappeared, while anti-HBs levels are yet undetectable; or (c) chronic HBV infection during which HBsAg is undetectable due to very low viraemia, a clinical phase recognized as occult HBV infection (14-17). Although many countries implement an anti-HBc test before blood transfusion, in an attempt to decrease the risk of HBV transmission, recent studies (18-20) clearly demonstrated an absence of correlation between anti-HBc positivity and presence of HBV-DNA, indicating that anti-HBc screening is not a recommended method to identify HBV infection. For instance, a donor showing positivity for anti-HBc may be a naïve-HBV carrier i.e. a person with a past history of HBV infection or a carrier of non-detectable levels of HBsAg (14, 17, 21-24). The false positive indications obtained in anti-HBc test can lead to discard of large quantities of blood units as well as losing precious blood samples especially those issued from donors exhibiting rare blood types. More recently, triplex nucleic acid tests (NATs) have been implemented in many countries, including the United States and China, during blood screening for infectious agents such as HBV, HCV, and HIV. A major benefit of HBV NAT is the detection of OBI. Moreover, it has been shown that NAT and serological analysis are complementary during detection of HBV in first-time donors, while NAT is more beneficial in the case of repeat donors (25). Alternatively, this method is challenged by the different sensitivities of the existing commercial NAT assay systems (26-28). Nowadays, different approaches can be conceived to reduce the risk of transfusion-transmitted HBV. These vary depending on different parameters including: the socioeconomic situation in a certain country, the prevalence of HBV, and the availability of certain technical services. Hence, different algorithms can be followed, such as a sole serological screening for HBsAg, a test for both HBsAg and anti-HBc, NAT approach, or a combination of serology and NAT. The aim of the present work was to describe a new strategy that ensures blood safety and resolves the problem of false positive results obtained during anti-HBc screen method. During this study, the observed HBsAg-positivity rate (0.8%) was comparable to that identified previously in Lebanese population (0.98% in 1998; 0.75% in 1999; 0.78% in 2000; 0.77% in 2001; 0.66% in 2002, and 0.6% in 2003) (21). The major output of this study was that HBsAg-negative but anti-HBc-positive blood samples, identified among 7200 tested samples, were classified into 2 categories. The first category included anti-HBc positive samples that were positive for anti-HBs. All Samples belonging to this category were negative for HBV-DNA. The second category was composed of anti-HBc positive samples that were negative for anti-HBs. Some of those samples were positive for HBV-DNA whilst others were negative. Interestingly, our observations indicated the association of the absence of HBV-DNA with the simultaneous presence of both anti-HBc and anti-HBs. Of great importance, our data indicates that a double anti-HBc and anti-HBs test would be beneficial to ensure blood safety and avoid loss of blood units. For instance, instead of directly discarding a blood sample showing positivity for anti-HBc, an additional screen for anti-HBs positivity will lead to keeping this sample. Hence, our proposed diagnosis strategy will be highly beneficial at both medical and socioeconomic levels.




  • 1.

    Lavanchy D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat. 2004; 11(2) : 97 -107 [DOI][PubMed]

  • 2.

    European Association For The Study Of The L. EASL clinical practice guidelines: Management of chronic hepatitis B virus infection. J Hepatol. 2012; 57(1) : 167 -85 [DOI][PubMed]

  • 3.

    Milich DR, Sallberg M, Maruyama T. The humoral immune response in acute and chronic hepatitis B virus infection. Springer Semin Immunopathol. 1995; 17(2-3) : 149 -66 [DOI][PubMed]

  • 4.

    Jung MC, Pape GR. Immunology of hepatitis B infection. Lancet Infect Dis. 2002; 2(1) : 43 -50 [DOI][PubMed]

  • 5.

    Laperche S, Guitton C, Smilovici W, Courouce AM. Blood donors infected with the hepatitis B virus but persistently lacking antibodies to the hepatitis B core antigen. Vox Sang. 2001; 80(2) : 90 -4 [DOI][PubMed]

  • 6.

    Chan HL. Changing scene in hepatitis B serology interpretation. Hosp Med. 2002; 63(1) : 16 -9 [DOI][PubMed]

  • 7.

    Said ZN. An overview of occult hepatitis B virus infection. World J Gastroenterol. 2011; 17(15) : 1927 -38 [DOI][PubMed]

  • 8.

    Ramezani A, Banifazl M, Eslamifar A, Aghakhani A. Serological pattern of anti-HBc alone infers occult hepatitis B virus infection in high-risk individuals in Iran. J Infect Dev Ctries. 2010; 4(10) : 658 -61 [PubMed]

  • 9.

    Kang SY, Kim MH, Lee WI. The prevalence of "anti-HBc alone" and HBV DNA detection among anti-HBc alone in Korea. J Med Virol. 2010; 82(9) : 1508 -14 [DOI][PubMed]

  • 10.

    Nanu A, Sharma SP, Chatterjee K, Jyoti P. Markers for transfusion-transmissible infections in north Indian voluntary and replacement blood donors: prevalence and trends 1989-1996. Vox Sang. 1997; 73(2) : 70 -3 [DOI][PubMed]

  • 11.

    Yotsuyanagi H, Yasuda K, Moriya K, Shintani Y, Fujie H, Tsutsumi T, et al. Frequent presence of HBV in the sera of HBsAg-negative, anti-HBc-positive blood donors. Transfusion. 2001; 41(9) : 1093 -9 [DOI][PubMed]

  • 12.

    Huang CF, Lin SS, Ho YC, Chen FL, Yang CC. The immune response induced by hepatitis B virus principal antigens. Cell Mol Immunol. 2006; 3(2) : 97 -106 [PubMed]

  • 13.

    Galli C, Orlandini E, Penzo L, Badiale R, Caltran G, Valverde S, et al. What is the role of serology for the study of chronic hepatitis B virus infection in the age of molecular biology? J Med Virol. 2008; 80(6) : 974 -9 [DOI][PubMed]

  • 14.

    Kleinman SH, Kuhns MC, Todd DS, Glynn SA, McNamara A, DiMarco A, et al. Frequency of HBV DNA detection in US blood donors testing positive for the presence of anti-HBc: implications for transfusion transmission and donor screening. Transfusion. 2003; 43(6) : 696 -704 [DOI][PubMed]

  • 15.

    Kuhns MC, Busch MP. New strategies for blood donor screening for hepatitis B virus: nucleic acid testing versus immunoassay methods. Mol Diagn Ther. 2006; 10(2) : 77 -91 [DOI][PubMed]

  • 16.

    Weber B, Melchior W, Gehrke R, Doerr HW, Berger A, Rabenau H. Hepatitis B virus markers in anti-HBc only positive individuals. J Med Virol. 2001; 64(3) : 312 -9 [DOI][PubMed]

  • 17.

    Manzini P, Girotto M, Borsotti R, Giachino O, Guaschino R, Lanteri M, et al. Italian blood donors with anti-HBc and occult hepatitis B virus infection. Haematologica. 2007; 92(12) : 1664 -70 [DOI][PubMed]

  • 18.

    Jutavijittum P, Andernach IE, Yousukh A, Samountry B, Samountry K, Thammavong T, et al. Occult hepatitis B infections among blood donors in Lao PDR. Vox Sang. 2014; 106(1) : 31 -7 [DOI][PubMed]

  • 19.

    Pisano MB, Blanco S, Carrizo H, Re VE, Gallego S. Hepatitis B virus infection in blood donors in Argentina: prevalence of infection, genotype distribution and frequency of occult HBV infection. Arch Virol. 2016; 161(10) : 2813 -7 [DOI][PubMed]

  • 20.

    Karimi G, Zadsar M, Vafaei N, Sharifi Z, FalahTafti M. Prevalence of antibody to Hepatitis B core antigen and Hepatitis B virus DNA in HBsAg negative healthy blood donors. Virol J. 2016; 13 : 36 [DOI][PubMed]

  • 21.

    Ramia S, Ramlawi F, Kanaan M, Klayme S, Naman R. Frequency and significance of antibodies against hepatitis B core (anti-HBc) antigen as the only serological marker for hepatitis B infection in Lebanese blood donors. Epidemiol Infect. 2005; 133(4) : 695 -9 [DOI][PubMed]

  • 22.

    Katz L, Strong DM, Tegtmeier G, Stramer S. Performance of an algorithm for the reentry of volunteer blood donors deferred due to false-positive test results for antibody to hepatitis B core antigen. Transfusion. 2008; 48(11) : 2315 -22 [DOI][PubMed]

  • 23.

    Altunay H, Kosan E, Birinci I, Aksoy A, Kirali K, Saribas S, et al. Are isolated anti-HBc blood donors in high risk group? The detection of HBV DNA in isolated anti-HBc cases with nucleic acid amplification test (NAT) based on transcription-mediated amplification (TMA) and HBV discrimination. Transfus Apher Sci. 2010; 43(3) : 265 -8 [DOI][PubMed]

  • 24.

    Dreier J, Kroger M, Diekmann J, Gotting C, Kleesiek K. Low-level viraemia of hepatitis B virus in an anti-HBc- and anti-HBs-positive blood donor. Transfus Med. 2004; 14(2) : 97 -103 [DOI][PubMed]

  • 25.

    Lelie N, Bruhn R, Busch M, Vermeulen M, Tsoi WC, Kleinman S, et al. Detection of different categories of hepatitis B virus (HBV) infection in a multi-regional study comparing the clinical sensitivity of hepatitis B surface antigen and HBV-DNA testing. Transfusion. 2017; 57(1) : 24 -35 [DOI][PubMed]

  • 26.

    Kiely P, Margaritis AR, Seed CR, Yang H, Australian Red Cross Blood Service NG. Hepatitis B virus nucleic acid amplification testing of Australian blood donors highlights the complexity of confirming occult hepatitis B virus infection. Transfusion. 2014; 54(8) : 2084 -91 [DOI][PubMed]

  • 27.

    Enjalbert F, Krysztof DE, Candotti D, Allain JP, Stramer SL. Comparison of seven hepatitis B virus (HBV) nucleic acid testing assays in selected samples with discrepant HBV marker results from United States blood donors. Transfusion. 2014; 54(10) : 2485 -95 [DOI][PubMed]

  • 28.

    Lin H, Zhao H, Tang X, Hu W, Jiang N, Zhu S, et al. Serological patterns and molecular characterization of occult hepatitis b virus infection among blood donors. Hepat Mon. 2016; 16(10) : 40492

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