Genotype of Hepatitis B Virus Isolates from Iranian Chronic Carriers of the Virus


Maryam Vaezjalali 1 , Seyed Moayed Alaviyan ORCID 2 , * , Seyed Mohammad Jazayeri 1 , Rakhshandeh Nategh 1 , Mahmood Mahmoodi 1 , Bashir Hajibeigi 1 , Talat Mokhtari Azad 1

1 Department of Virology, Faculty of Public Health, Medical Sciences/University of Tehran, IR-Iran

2 Baqiyatallah Research Center for Gastroenterology and Liver Disease (BRCGL), Baqiyatallah University of Medical Sciences, [email protected], IR-Iran

How to Cite: Vaezjalali M, Alaviyan S, Jazayeri S, Nategh R, Mahmoodi M, et al. Genotype of Hepatitis B Virus Isolates from Iranian Chronic Carriers of the Virus, Hepat Mon. Online ahead of Print ; 8(2):97-100.


Hepatitis Monthly: 8 (2); 97-100
Article Type: Research Article
Received: September 16, 2007
Accepted: February 16, 2008


Background and Aims: Determination of the genotypes and subtypes of hepatitis B virus (HBV) provides epidemiological data, which can contribute further to vaccination and antiviral treatment strategies, diagnostic development, and prediction of the disease course. The aim of this study was to describe the molecular characterization and phylogenetic analysis of 60 HBV S-region isolated from native Iranian patients with chronic HBV infection.

Methods: HBV-positive sera were collected from Iranian patients with hepatitis B infection in Tehran Hepatitis Center. HBV-DNA was extracted and the partial HBV S ORF (677bp) were chosen for amplification and sequencing.

Results: By comparing the sequences of HBV isolated from Iranian patients with 50 complete sequences of HBV retrieved from the GenBank database, representing all other existing genotypes, the sequences of all 60 patients were consistent with that of genotype D. All HBV isolates from Iranian patients were clustered in genotype D branch with high bootstrap values.

Conclusions: Sixty HBV isolates from Iranian patients with chronic hepatitis B represent homogenous genotypic diversity. These sequences of Iranian HBV genomes may contribute to the information on the genetic diversity of HBV worldwide.

© 0, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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