The effect of L-Arginine supplementation on Delayed Onset Muscle Soreness (DOMS) after eccentric heavy exercise


Babak Nakhostin-Roohi 1 , * , Zahra Mohammadi Aghdam 2

1 Department of Physical Education and Sport Sciences, Ardabil Branch, Islamic Azad University, Ardabil, Iran.

2 Department of Physical Education, University of Mohaghegh-Ardabili, Ardabil, Iran.

How to Cite: Nakhostin-Roohi B , Mohammadi Aghdam Z . The effect of L-Arginine supplementation on Delayed Onset Muscle Soreness (DOMS) after eccentric heavy exercise, Hormozgan Med J. 2017 ; 21(3):e87323. doi: 10.29252/hmj.21.3.169.


Hormozgan Medical Journal: 21 (3); e87323
Published Online: September 24, 2017
Article Type: Research Article
Received: July 11, 2017
Accepted: September 24, 2017


Introduction: L-Arginine supplementation improves antioxidant defenses through LArginine/
nitric oxide pathways. This investigation assesses the influences of acuteLArginine
supplementation on selected markers of delayed onset muscle soreness (DOMS)
after one by one heavy eccentric exercise in healthy young females.
Methods: Twelve healthy young females students of the University of Mohaghegh-Ardabili
voluntarily participated in a double-blind randomized-controlled crossover trial to estimate the
effects of 3g L-Arginine oral supplementation versus placebo (3g glucose) following an
unaccustomed heavy eccentric exercise. L-Arginine (ARG) or placebo (PLC) was taken
immediately after squat exercise, separated by 10day washout. Measurements were conducted
at baseline, 24, and 48h after exercise comprising: (a) TAC (b) CK (c) LDH (d) Visual
Analogue Scale (VAS) (e) Range of Motion (ROM) of both knees (f) Edema of both thighs.
For data analysis software SPSS version 20 was used. To analysis the data, analysis of
variance with repeated measure was used.
Results: TAC significantly increased 48h after exercise compared with the pre-exercise
just in ARG group (P<0.05). CK and LDH significantly enhanced 24, and 48h after
exercise only in the PLC group (P<0.05). VAS and edema increased 24, and 48h after
exercise compared with the pre-exercise in both groups (P<0.05). Moreover, ROM
decreased in both groups 24, and 48h after exercise compared with the pre-exercise
(P<0.05). There were no between-group differences in any time series (P>0.05).
Conclusion: The findings of this study suggest that acute supplementation of ARG after
heavy eccentric exercise may alleviate muscle damage through promoting the antioxidant
capacity, protein synthesis, or decrease of lactate accumulation.



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