Hypercholesterolemia role of vitamin D3 and genistein effect in reducing atherosclerosis in rat


Azam Mansouri 1 , Akbar Vahdati 2 , Mehdi Nematbakhsh 3 , Jamal Moshtaghian 4 , *

1 PhD of Physiology, Farhangian University, Tehran, Iran.

2 Professor Department of Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Professor Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

4 Associate Professor Department of Biology, Isfahan University, Isfahan, Iran.

How to Cite: Mansouri A , Vahdati A , Nematbakhsh M , Moshtaghian J . Hypercholesterolemia role of vitamin D3 and genistein effect in reducing atherosclerosis in rat, Hormozgan Med J. 2015 ; 19(5):e87610.


Hormozgan Medical Journal: 19 (5); e87610
Published Online: November 13, 2014
Article Type: Research Article
Received: April 26, 2014
Accepted: November 13, 2014


Introduction: Atherosclerosis has been identified as the most agents for premature death
and disability and of its injuries is growing masses of fatty materials with fibrous
connective tissue and deposition of calcium salts and other blood materials. To do
research on atherosclerosis, in creating hypercholesterolemia animal model is important.
Methods: In this wxperimental study, 4 groups of rats were used (n=12). Group 1 and
Group 2 received food-based dietary and Group 2 addition of it, received 2% cholesterol
and 0.5% colic acid. Groups 3 and 4 in addition to the group 2 food, respectively.
Vitamin D 3 (300000IU/Kg BW) and vitamin D2 (15000IU/Kg BW), received group 3
had the highest cholesterol levels after 6 weeks that were distributed to 3 subtypes A, B
and C. A subgroup received previous diet, and subtypes B and C, in addition to the
previous feed, respectively received genistein with doses 1.5 and 3 mg/kg BW for 6
weeks. For statistical analysis of the data ANOVA and LSD tests were used and P<0.05
was considered significant.
Results: In the first stage there were significant differences in cholesterol, LDL,
LDL/HDL and atherogenic index between experimental and control groups and also
between group 3 and other two treatment groups that is the effect of vitamin D for
hypercholesterolemia model. The Second stage, revealed the effect of genistein to reduce
the cardiovascular damage.
Conclusion: Vitamin D3 is one of atherogenic factors that if it be accompanied with high
cholesterol, can be caused atherosclerosis similar to human that can be used for studying
related to atherosclerosis. Types of isoflavones in diets can reduce atherosclerotic damages
due of vitamin D3 accompanied with high cholesterol.



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