Comparison of Essential and Non Essential Amino Acids in the Microbial Protein of Pleurotus Florida from the Lignocellulosic Wastes


Jhaleh khanifar 1 , * , Alireza Ahmadi 2 , Hedayatallah Ghoorchian 3 , Reza Haji Hosseini 4 , Mohammad Hasan Sheikhha 5 , bibiFatemeh Haghirosadat 6

1 MSc of Biochemistry, Payamenoor University, Tehran, Iran.

2 Assistant Professor Department of Biomedical, Alzahra University, Tehran, Iran.

3 Associate Professor Department of Biophysics, Payamenoor University, Tehran, Iran.

4 Associate Professor Department of Biochemistry, Tehran University, Tehran, Iran.

5 Assistant Professor Department of Genetics, Shahid Sadughi University of Medical Sciences, Yazd, Iran.

6 PhD of Nano Biotechnology, Tehran University , Tehran, Iran.

How to Cite: khanifar J, Ahmadi A, Ghoorchian H, Haji Hosseini R, Sheikhha M H, et al. Comparison of Essential and Non Essential Amino Acids in the Microbial Protein of Pleurotus Florida from the Lignocellulosic Wastes, Hormozgan Med J. 2013 ; 17(2):e88089.


Hormozgan Medical Journal: 17 (2); e88089
Published Online: January 30, 2012
Article Type: Research Article
Received: July 23, 2011
Accepted: January 30, 2012


Introduction: Cereal straws contain Cellulose, Hemicelluloses and Lignin and are most
available renewable biopolymers. White rot fungi is used to convert these wastes into
microbial protein. Pleurotus Florida are having the most delignification ability amongst other
micro-organisms. We determined the amounts of protein, essential and non essential amino
acids of the produced microbial protein from the wheat straw.
Methods: Wheat straw was pretreated with NaOH 2% at 1000C temperature on autoclave
condition. Then it was inoculated with Pleurotus Florida which provided by Mandel's media
with 0.3 g/lit Urea and incubated for 4 weeks under room temperature. Protein
concentration of microbial protein was determined. The extracted protein partly hydrolyzed
with HCl 6 Normal and the other part hydrolyzed with Ba(OH)2 4 Normal for 48 hours
under 1100C temperature condition and its amino acids analyzed by using A-200 Amino
Nova analyzer.
Results: The amount of protein extracted after 4 full test runs were 62.8 % per 100 g of dried
microbial protein and the profile concentration of Non_essential and Essential amino acids
was: Aspartic acid=5.22, Serine=3.6, Glutamic acid=6.38, Prolin=3.2, Glycine=4.21,
Alanine=6.23, Cycteine=1.18, Tyrosine=2.61 Threonine=0.6, Valine=6.6,
Methionine=2.1, Isoleucine=7.3, Leucine=6.8, Phenylalanine=4.3, Histidine=19.8,
Lysine=9.5, Arginine=8.3, Tryptophan=0.96 g/100g of extracted protein. The ratio of
essential amino acids to the total amino acids was 65.6%.
Conclusion: Eessential and non essential amino acids obtained are indicative of its high
quality. In the microbial protein produced by our study, considerable amounts of amino
acids such as Lysine, Histidine, Lucine, Isolucine, Alanine, Arginine and Tryptophan were
detected and as such it can be a proper replacement for the current available animal feed in
the market.



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