Effect of endogenous nitric oxide on cardiac ischemic preconditioning in rat


Fatemeh Mirershadi 1 , * , Mahdiyeh Faghihi 2 , Ahmadreza Dehpour 3

1 Instructor Department of Physiology, Islamic Azad University Ardabil Branch, Ardabil, Iran.

2 Associate Professor Department of Physiology, Tehran University of Medical Sciences, Tehran, Iran.

3 Professor Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran.

How to Cite: Mirershadi F, Faghihi M, Dehpour A. Effect of endogenous nitric oxide on cardiac ischemic preconditioning in rat, Hormozgan Med J. 2010 ; 14(1):e88531.


Hormozgan Medical Journal: 14 (1); e88531
Published Online: October 31, 2009
Article Type: Research Article
Received: May 24, 2009
Accepted: October 31, 2009


Introduction: Ischemic Preconditioning (IPC) is the phenomen that happens on the heart
by one or several short periods of ischemia followed by reperfusion that improve the
postischemic recovery of mechanical function. Ischemic preconditioning (IPC) may
protect the heart from ischemia reperfusion injury by nitric oxide formation. This study
investigated the effect of ischemic preconditioning on heart and the relationship between
nitric oxide.
Methods: 28 male Sprague dawley rats (200-250 g) in Tehran University of Medical
Sciences were used. Rats were anesthetized and hearts were rapidly isolated and perfused
in the Langendorff mode at a constant perfusion pressure and temperature of 37 ̊C. hearts
were divided to 4 groups. Control group was perfused 170 minutes with buffer solution.
ischemic reperfusion (IR) group was subjected to 30 minutes ischemia. (Ischemic
preconditioning) IPC group was elicited by 5 min ischemia followed 5 min reperfusion
before IR and L-NAME + IPC group, L-NAME (0.1mM) was added into the perfusion
solution. Heart rate (HR), left ventricular development (LVDP), RPP (LVDP × HR),
infarct size and coronary flow (CF) were measured. ANOVA tests( with TUKEY post
test if p<0.05) were used for statistical analyses.
Results: IPC improve the postischemic recovery and reduced postischemic ventricular
dysfunction in heart and reduction of infarction size. No significant differences were
observed between IPC and IPC + L-NAME groups.
Conclusion: L-NAME did not affect postischemic recovery of IPC so in the isolated
heart NO isn't involved in the cardioprotective effect of IPC.



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