Detection of Plasmodium Vivax by Nested PCR in marious areas of western north of Iran


Abbas Shahbazi 1 , * , Ahmad Raeisi 2 , Mehdi Asmar 3 , Saeid Naddaf 3 , Mehdi Nateghpour 4 , Gholamreza Anaraki 5 , Mahmoud Mahami 6

1 Tabriz Research Center of Infectious & Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.

2 Center of Diseases Management, Ministry of Health, Tehran, Iran.

3 Department of Parasitology, Pastor Institute of Iran, Tehran, Iran.

4 Associate Professor Department of Medical Parasitology & Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

5 Pathologist, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

6 PhD Student of Medical Parasitology, Tarbiat Modarres University, Tehran, Iran.

How to Cite: Shahbazi A, Raeisi A , Asmar M, Naddaf S, Nateghpour M, et al. Detection of Plasmodium Vivax by Nested PCR in marious areas of western north of Iran, Hormozgan Med J. 2009 ; 13(1):e88619.


Hormozgan Medical Journal: 13 (1); e88619
Published Online: October 28, 2008
Article Type: Research Article
Received: March 02, 2008
Accepted: October 28, 2008


Introduction: Malaria is one of the most important parasitic diseases in tropical and
sub-tropical areas of the world and kills a large number of People annually. Diagnosis of
the disease is preformed by microscopic and molecular methods. This study has been
designed for detecting of latent/sub-patent infection caused by plasmodium vivax in
individuals with history of vivax malaria without any clinical signs by means of
microscopic and molecular methods.
Methods: In this descriptive study, the blood samples of 38 individuals from Parsabad
(Ardabil province) and Kaleiber (East Azarbayejan province) has been collected one year after
primary diagnosis of vivax malaria and effective treatment of the diseases, based on the
standard country's protocol, as well as upon primary microscopic detection of thin and thick
blood smears. They were assessed by Nested-PCR method in Pastor Institute of Iran.
Results: All samples were negative by microscopic method; we found one vivax positive
case via Nested-PCR.
Conclusion: Microscopic examination is a selective and standard method in malaria
diagnosis. Due to the probability of existence of latent/sub-patent infection in endemic areas
and microscopic misdiagnosis of thin and thick blood smears and epidemiologic importance of
malaria diagnosis, the use of molecular methods such as Nested-PCR in individuals with
history of vivax malaria to detect any latent/sub/patent infection seems to be beneficial.



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