Isolation and characterization of bacteria and fungi in ambient air of Qeshm Island


babak Barati 1 , * , Mohammad Ghahri 2 , Rahim Soruri 1

1 Research Center of Applied Biotechnology, Baghiyatalla University of Medical Sciences, Tehran, Iran.

2 Instructor Department of Biology, Imam Hossein University, Tehran, Iran.

How to Cite: Barati B, Ghahri M, Soruri R. Isolation and characterization of bacteria and fungi in ambient air of Qeshm Island, Hormozgan Med J. 2009 ; 13(2):e88644.


Hormozgan Medical Journal: 13 (2); e88644
Published Online: December 28, 2008
Article Type: Research Article
Received: March 16, 2008
Accepted: December 28, 2008


Introduction: Many studies have shown an association between respiratory distress,
allergic reactions and the presence of bacteria and fungi in air. We aimed to compare
the air micro flora of Qeshm Island with those of other provinces.
Methods: This experimental study was consisted of grown colonies on PDA, SC
media for fungi and BHI, TSA media for bacteria. Samples were taken by Air
Trapping and sedimentation from respiratory plane level. The colonies were grown in
nutrient media. After incubation, they were examined through microscopic as well as
macroscopic, and then were counted. Isolated Bacteria were identified by
biochemical tests. The molds were identified by slide culture and teased mount.
Results: Of 913 bacterial colonies that were isolated, 559 colonies were gram
positive bacillus, the highest in number. 254 colonies were gram positive cocci,
the second class of isolated bacteria. Bacillus sp. 359 (39.33%) were the most
numerous genus of bacteria in the air of Qeshm Island.
Conclusion: Resistance of gram positive bacteria to dry environmental condition and
the sporogenesis of bacillus enabling survival at harsh conditions were the main reasons
of high rate of bacillus isolated.



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