The effects of vitex agnus castus extract and interaction with bromocriptine on luteinizing hormone and testosterone in male mice


Sima Nasri 1 , * , Shahrbanoo Orian 2 , Ali Haeri Rohani 3 , Gholamreza Amin 4 , Mokhtarali Naghizadeh 5

1 Assistant professor, Department of Biology, Azad Eslami University Parand branch, Tehran, Iran.

2 Full professor, Department of Biology Training & Education, University of Tehran, Tehran, Iran.

3 Full professor, Department of physiology, Tehran University, Tehran, Iran.

4 Assistant professor, Department of Pharmacogenozy, Tehran University of Medical Sciences, Tehran, Iran.

5 Master of Hematology, Oroomieh Blood Transfusion Organization.

How to Cite: Nasri S, Orian S, Haeri Rohani A, Amin G, Naghizadeh M. The effects of vitex agnus castus extract and interaction with bromocriptine on luteinizing hormone and testosterone in male mice, Hormozgan Med J. 2005 ; 9(2):e90344.


Hormozgan Medical Journal: 9 (2); e90344
Published Online: May 17, 2005
Article Type: Research Article
Received: January 16, 2005
Accepted: May 17, 2005


Introduction: Several clinical studies have confirmed the beneficial effect of
plants extracts in the treatment of disorders. The purpose of this study was to
evaluate the probable effects of vitex agnus–castus (VAC) on the male
reproductive physiology, and also the dopaminergic agonist (Bromocriptine) on
male mice.
Methods: In this experimental study, in order to evaluate, the medicinal effects
of vitex agnus castus, we used the hydroalcoholic VAC. Extract injection with
the following doses: 65, 165, 265,365 and 465 mg/kg; bromocriptine as a
dopamine agonist receptor (5,10,20 mg/kg). To study the interaction between
VAC. Extract and bromocriptine, we injected the optimum doses of VAC. with
bromocriptine at the same time. Intraperitoneal injections were applied in all
experiments, once a day for 30 days. The control group remained intact and the
sham group received vehicle. After the last injection, we collected the animal
blood serums for hormonal assays. For statistical analysis, analysis of variance
was used and P<0.05 was considered significant.
Results: LH and testostrone showed significant decrease in bromocriptine group.
VAC. Extract decreased significantly the LH and Testosterone levels. The
coadministration of VAC. Extract and bromocriptine decreased LH and
Conclusion: These results suggest: Dopamine regulates the gonadotroph – Leyding
cells axis. It appears that VAC. Extract effects through dopaminergic system and
other pathways.



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