Loss of P16 Protein Expression and Its Association with Epstein‐Barr Virus LMP‐1 Expression in Hodgkin's Lymphoma


Fawzi Irshaid 1 , * , Khaled Tarawneh 2 , Aisha Alshdefat 3 , Fatiha Dilmi 1 , Adnan Jaran 1 , Raji Al‐Hadithi 4 , Ahad Al‐Khatib 4

1 Dept. of Biological Sciences, Faculty of Science, Al al-Bayt University, Al-Mafraq, Jordan

2 Dept. of Biological Sciences, Faculty of Science, Mutah University, Mutah, Jordan

3 Mafraq Hospital of Children and Maternity, Al-Mafraq, Jordan

4 Dept. of Pathology, Faculty of Medicine, Jordan University of Sciences and Technology, Irbid, Jordan

How to Cite: Irshaid F , Tarawneh K, Alshdefat A, Dilmi F, Jaran A, et al. Loss of P16 Protein Expression and Its Association with Epstein‐Barr Virus LMP‐1 Expression in Hodgkin's Lymphoma, Int J Cancer Manag. 2013 ; 6(2):e80404.


International Journal of Cancer Management: 6 (2); e80404
Published Online: June 30, 2013
Article Type: Research Article
Received: January 29, 2013
Accepted: February 23, 2013


Background: Expression of Epstein-Barr virus Latent Member Protein-1 (EBV LMP1) and loss of P16 protein expression are documented in lymphoma, indicating a relationship between them, but this relationship is not clear and sometimes contradictory. Thus, this study was conducted to examine the relationship between the loss of P16 and EBV LMP-1 expression in Jordanian patients diagnosed with lymphoma.

Methods: Sections were made from archival formalin-fixed and paraffinembedded blocks from 55 patients diagnosed with lymphoma. P16 expression and LMP-1 expression were detected by immunohistochemistry using monoclonal antibodies.

Results: In Hodgkin's Lymphoma (HL), the loss of P16 was higher in LMP-1 positive cases (61%) than LMP-1 negative cases (25%; P = 0.072). Conversely, in NonHodgkin's Lymphoma (NHL), none of LMP-1 positive samples showed loss of P16. Furthermore, among LMP-1 HL positive cases, the loss of P16 was more frequent in male (75%) than female (33%). Also, there was a significantly higher proportion of LMP-1 positive cases showing loss of P16 in HL (11:18), compared to those in NHL (0:8, P < 0.001), confirming a difference between HL and NHL, concerning the LMP-1/P16 relationship.

Conclusion: A trend for an association between loss of P16 and LMP-1 expression was observed in HL but not NHL patients. These findings suggest that there are molecular and clinical differences in the pathogenesis and development of different subtypes of lymphoma.

© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.


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