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Flow Cytometry Analysis of Rosa Damascena Effects on Gastric Cancer Cell Line (MKN45)

AUTHORS

Hanieh Khatib 1 , Mostafa Rezaei‐Tavirani 2 , Saeed Heidari Keshel 2 , Mona Zamanian Azodi 2 , Roghiyeh Omidi 2 , Mohsen Biglarian 2 , Sara Sobhi 3 , *

1 Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

How to Cite: Khatib H, Rezaei‐Tavirani M, Heidari Keshel S, Zamanian Azodi M, Omidi R, et al. Flow Cytometry Analysis of Rosa Damascena Effects on Gastric Cancer Cell Line (MKN45), Int J Cancer Manag. 2013 ; 6(Supplement):e80457.

ARTICLE INFORMATION

International Journal of Cancer Management: 6 (Supplement); e80457
Published Online: December 30, 2013
Article Type: Research Article
Received: November 08, 2012
Accepted: December 25, 2012
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Abstract

Background: Gastric cancer is a life threatening disease. Recent studies have shown that Rosa Damascene possesses noticeable biological effects on the human body. This study, investigates the anticancer effect of Rosa Damascena on gastric cancer cell line MKN45.

Methods: Microscopic studies and MTT assay were used to examine morphological alteration, and cell survival of the cancer cells while exposing to differentvolumes of the essential oilrespectively. In addition, flow cytometry was applied to determine the cell death mechanism of the gastric cancer treated with the essential oil.

Results: The findings indicate that Rosa Damascena essential oil affects gastric cancer cells in two distinctive ways: the soluble phase increases cell viability, while the vapor phase decreases cellsurvival. Moreover, flow cytometry analysis revealed that apoptosis is the main mechanism accompanied with cell death.

Conclusion: Consequently, Rosa Damascena essential oil can be used as a potent anticancer agent in the future. However,more evaluation of this essential oil is still needed to elicit its effectivebiological activities

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© 2013, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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