Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat


Mohammad Reza Aliparasti 2 , Mohammad Reza Alipour 3 , Shohreh Almasi 2 , Hadi Feizi 4 , *

2 Department of Immunology, Tabriz University of Medical Sciences, IR Iran

3 Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, IR Iran

4 Department of Physiology, Hormozgan University of Medical Sciences, [email protected], IR Iran

How to Cite: Aliparasti M R, Alipour M R, Almasi S, Feizi H. Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat, Int J Endocrinol Metab. Online ahead of Print ; 10(3):553-557. doi: 10.5812/ijem.3914.


International Journal of Endocrinology and Metabolism: 10 (3); 553-557
Published Online: June 30, 2012
Article Type: Original Article
Received: December 19, 2011
Accepted: March 13, 2012


Background: Chronic hypoxia causes apoptosis of cardiac myocytes, however, energy production by anaerobic glycolysis protects myocardium against hypoxia injuries. Aldolase A is a well-characterised key enzyme of the glycolysis pathway. Ghrelin, a 28-amino-acid peptide, synthesizes in the stomach and has protective roles in cardiovascular systems and also affects metabolic pathways.

Objectives: Therefore, the aim of this study was to evaluate the effect of ghrelin on aldolase A gene expression after chronic hypoxia in the rat hearts.

Materials and Methods: Twenty four adult male wistar rats were randomly divided into three groups. Hypoxic rats with saline or ghrelin treatment were placed in a normobaric hypoxic chamber (O2 11 %), for two weeks. Controls remained in room air. Aldolase A gene expression was measured by Real-Time RT-PCR.

Results: the transcriptiom rate of Aldolase A in hypoxic animals did not change significantly compared to negative control ones. During chronic hypoxia, ghrelin treatment increased the amount of heart Aldolase A gene expression compared to negative controls (P = 0.029). Hypoxic animals that were treated with ghrelin were significantly more polycythemic than the controls and even hypoxic with saline treated rats (P < 0.001).

Conclusions: It seems that ghrelin interferes in the cardiac metabolism through up-regulation of glycolytic enzymes. In other words, it may protect heart from possible hypoxia induced damages.

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