Liver and Plasma Nesfatin-1 Responses to 6 Weeks of Treadmill Running With or Without Zizyphus Jujuba Liquid Extraction in Female Rat


Abbass Ghanbari Niaki 1 , * , Fatemeh Mohammadi Joojadeh 1 , Navabeh Zare Kookandeh 1 , Safar Najafi 1 , Mohammad Javad Chaichi 2 , Fatemeh Rodbari 3 , Hasan Bayat 4

1 Exercise Biochemistry Division, Department of Physical Education and Sport Sciences, University of Mazandarn, Baboulsar, IR Iran

2 Department of Analytical Chemistry, Faculty of Chemistry, Mazandarn University, Baboulsar, IR Iran

3 Department of cell and molecular biology, Faculty of Basic Sciences, University of Mazandran, Baboulsar, IR Iran

4 Sina Laboratory, Mazandaran, Ghaemshahr, IR Iran

How to Cite: Ghanbari Niaki A, Mohammadi Joojadeh F, Zare Kookandeh N, Najafi S, Chaichi M J, et al. Liver and Plasma Nesfatin-1 Responses to 6 Weeks of Treadmill Running With or Without Zizyphus Jujuba Liquid Extraction in Female Rat, Int J Endocrinol Metab. Online ahead of Print ; 11(2):95-101. doi: 10.5812/ijem.8438.


International Journal of Endocrinology and Metabolism: 11 (2); 95-101
Published Online: March 31, 2013
Article Type: Original Article
Received: October 11, 2012
Accepted: February 12, 2013


Background: Nesfatin-1 is a protein derived from a precursor molecule of the nucleobindin-2 gene, and acts as an anorexigenic peptide on food intake behavior, and its level isinfluenced by nutritional status, food composition [fat and carbohydrate (CHO)], and physical exercise.

Objectives: The aim of this study was to investigate the effects of 6 weeks of treadmill running (at high intensity) program with and without zizyphus jujuba (high carbohydrate content) crud extraction on liver nesfatin-1, ATP, glycogen, and its plasma concentrations in female rats.

Materials and Methods: Twenty-eight Wistar female rats (6-8 weeks old100-120 g of weight) were randomly assigned to saline-control (SC), saline-training (ST), zizyphus jujuba-control (ZJC), and zizyphus jujuba-training (ZJT) groups. Rats ran on a motor-driven treadmill at 35 m/min, 60 min/day, 5 days/week for 6 weeks. Animals received ZJ extraction and saline at the dose of 1.25mL/100g of body weight. Seventy-two hours after the last training session rats were killed, a portion of liver excited, and plasma was collected for nesfatin-1, ATP, and glycogen measurements. A one-way ANOVA method, and Pearson correlation were employed. P < 0.05 was considered as significant.

Results: A higher and significant liver nesfatin-1 level was found in ZJ groups (p < 0.005), but plasma nesfatin-1 responded differently. Changes in liver nesfatin-1 were accompanied with an increase in liver glycogen,but not ATP contents.

Conclusions: The Findings indicate that higher liver nesfatin-1 and glycogen content by ZJ extraction might be due to the ZJ high CHO content, and it could be consideredas an anti-appetite herb.

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