Toxoplasmosis in Pregnant Women and Non-pregnant Female Volunteers in Port Harcourt, Rivers State, Nigeria

AUTHORS

Evelyn O Onosakponome 1 , Austin E Abah ORCID 2 , * , Michael Wogu ORCID 2

1 Department of Medical Laboratory Science, Pamo University of Medical Sciences, Port Harcourt, Nigeria

2 Department of Animal and Environmental Biology, University of Port Harcourt, Port Harcourt, Nigeria

How to Cite: Onosakponome E O, Abah A E, Wogu M. Toxoplasmosis in Pregnant Women and Non-pregnant Female Volunteers in Port Harcourt, Rivers State, Nigeria, Int J Infect. 2021 ; 8(2):e110598. doi: 10.5812/iji.110598.

ARTICLE INFORMATION

International Journal of Infection: 8 (2); e110598
Published Online: April 19, 2021
Article Type: Research Article
Received: November 7, 2020
Revised: January 3, 2021
Accepted: February 1, 2021
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Abstract

Background: Toxoplasmosis is caused by Toxoplasma gondii, a protozoan parasite, and is among the diseases generally referred to as neglected tropical disease (NTD). It can be a reason for impulsive abortion in pregnant women or hereditary chorioretinitis in neonates.

Objectives: The present study examined seroprevalence and related risk issues of toxoplasmosis in pregnant women (PTW) and non-pregnant females (NPF) of two health facilities in Port Harcourt.

Methods: Venous blood was collected from 380 women and analyzed by immunodiagnostic techniques using ELISA (Enzyme-Linked Immunosorbent assay) IgG and IgM tests and real-time polymerase chain reaction (PCR). Data were collected using a well-structured questionnaire on the socio-demographic risk issues linked with toxoplasmosis.

Results: Among the study population, PTW and NPF had seroprevalence of 33.0% and 19.4%, respectively (P < 0.05). PTW recorded a higher overall seroprevalence than NPF with 16.1%, 1.3%, and 8.4%, 0.8% for Toxoplasma gondii ELISA IgG, and IgM tests, respectively. IgM-positive cases were all authenticated by the real-time PCR technique and were all negative. Age group 35-39 years had the highest IgG seroprevalence of 24.3% for PTW, while the age group > 40 years had the highest IgG seroprevalence of 18.2% for NPF. Traders recorded the uppermost seroprevalence of 20.9% and 2.6% for IgG and IgM among PTW, respectively, while teachers and traders recorded the maximum seroprevalence of 15.6% and 1.7% for IgG and IgM among NPF, respectively. Statistical analysis showed that there was no association between the age group, occupation, trimester, and toxoplasmosis.

Conclusions: The toxoplasmosis was significantly high in pregnant women. It is recommended that Toxoplasma gondii tests should be included as a routine test during antenatal.

1. Background

Toxoplasmosis is a broad-based zoonosis disease caused by Toxoplasma gondii, which is an oblige parasite occurring within the cells whose transporter and definitive hosts are cats in the homes and other cats (1, 2). The infection can also spread congenitally to newborns from their mothers (3, 4). Major infection in pregnancy can cause spontaneous abortion, death shortly before and after birth, or abnormal organ development in the fetus, contingent on the stage of the pregnancy (4, 5). Toxoplasmosis developed in the first trimester and second trimesters of pregnancies commonly show the severity of illness in newborns, though infection through the placenta is least probable during the first trimester (5). Infections during pregnancy in one-third of cases can be acquired by the fetus through the placental blood as the tachyzoite, which is the invasive/aggressive form of the T. gondii, is capable of crossing the placenta (5). Each fetus that contacted T. gondii during the stages of pregnancy can develop inborn toxoplasmosis with substantial hereditary nerve and visual disorder (4). The definite pervasiveness of infection due to T. gondii in pregnancy so far is not known but differs from country to country. Topical studies estimated the prevalence by the enzyme-linked immunosorbent assay (ELISA) method. The frequency of IgG antibodies was 38% in Iran and 50% in Brazil, whereas the frequency of 4% was reported for IgM in Iran and 90% in Brazil (6, 7)). In Nigeria, a seroprevalence of 44% was recorded (8). During pregnancy, the risk elements for toxoplasmosis are not very clear as some authors implicated domestic cats, eating raw meat, contaminated vegetables (9, 10) which are at variance with other studies that could not find such a relationship (6, 9). Owing to the obstetric and congenital squeals, the uncovering of primary toxoplasmosis is most life-threatening in pregnancy.

2. Objectives

The aim of the present study was to determine the preponderance and risk factors of toxoplasmosis in pregnant women (PTW) and non-pregnant females (NPF) attending antenatal clinics of two tertiary hospitals in Port Harcourt.

3. Methods

3.1. Study Area

The two tertiary hospitals were: Port Harcourt University Teaching Hospital (UPTH) and Rivers State University Teaching hospital (RSUTH), both located at Rivers State. UPTH is located within latitude 4°90’ N and longitude 6°93’ E, while RSUTH is located within latitude 4°78 N and 7°01 E. They are Federal (UPTH) and State (RSUTH) governmental hospitals as well as among the biggest referral healthcare facilities in Niger-Delta Region, Nigeria.

3.2. Study Design

A total of 380 women participated in this study, 200 PTW and 180 NPF volunteers (which served as controls) were randomly selected for the study. HIV seropositive women were excluded from this study.

3.3. Data on Demography

Pre-tested questionnaires taking into consideration information about age, sex, livelihood, ownership of pets, and other possible factors were administered to respondents.

3.4. Ethical Consideration

Ethical approval was obtained from the Committees in charge of Ethical matters of Port Harcourt University Teaching Hospital and Hospital Management Board of Rivers State, as well as oral/written informed consent was obtained from all study participants.

3.5. Samples Collection and Serological Test

From each of the 380 women, 3 ml of venous blood was collected by venipuncture from February to August 2016. To obtain the required serum, the samples of blood collected were allowed to stand to clot and then centrifuged for 5 minutes at 1500 rpm. Sera were collected and kept at 2°C. The reagents, developing plate’s cards, and specimens were allowed to thaw and were brought to a temperature of 24°C. The samples were treated using Bio Check for IgG and IgM enzyme, immunoassay test kit specific for Toxoplasma Immunoglobulins using standard laboratory methods.

3.6. Real-time PCR Technique

Commercial Toxoplasma gondii real-time PCR reagent with DNA extraction kit (by Liferiver Shanghai Zhijiang BiotechnologyCo. Ltd www.liferiverbiotech.com) was used according to the manufacturer’s procedure to extract DNA from the samples. The Real-time PCR method was applied to all samples using commercial standard T. gondii gene primers provided by the manufacturer (Liferiver Biotech) and standard recommended methods. Optimization studies were performed to determine the synthesized primers and best study concentrations for the probe. According to the results of this study, for 35 µL reaction mix, reaction volume was set with LightCycler probe mastermix 1 µL of each primer (10 pM), probe (4 pM) of 0.4 µL, molecular grade water of 5.6 µL, and DNA of 2 µL. The amplification cycles of PCR were as follows: 5 minutes at 95°C for initial denaturation, 40 cycles of two stages 15 seconds at 95°C for denaturation, and 30 seconds at 60°C for annealing-extension in a LightCycler Real-time PCR device (Roche, Mannheim, Germany) 10.

3.7. Data Analysis

Two-way factor analysis of variance (ANOVA) was used to analyze the data obtained from this study. Differences were considered to be significant when P < 0.05.

4. Results

The overall seroprevalence of toxoplasmosis in 380 serum samples was 101 (26.6%); PTW and NPF recorded seroprevalence of 61 (16.1%) and 32 (8.4%), respectively for IgG as well as 5 (1.3%) and 3 (0.8%) for IgM (Table 1), respectively. IgM-positive cases were all authenticated by PCR and were all negative. Figure 1 shows the result of the PCR spectral analysis for samples tested. Age groups 35 - 39 and > 40 showed the highest IgG seroprevalence of 17 (24.3%) and 4 (18.2%) in PTW and NPF, respectively (Table 1). The difference in the rate of seropositivity to T. gondii antibodies was not significant based on occupation, educational status, and trimester (Table 1). Table 2 shows the risk factors associated with seroprevalence that affect the transmission of T. gondii in the study population. Washing fruits before consumption and drinking treated water had a significant impact on the seroprevalence of toxoplasmosis.

Table 1. Toxoplasmosis Seroprevalence Based on Individual and Socio-Demographic Factors in Port Harcourt (P < 0.05)
Factors/ParametersNumber ExaminedNumber Positive (%)
PTWNPF
IgGIgMPCRIgGIgM
Overall38061 (16.1)5 (1.3)0 (0.0)32 (8.4)3 (0.8)
Age group
20 - 24406 (15.0)2 (5.0)2 (5.0)0 (0.0)
25 - 2912012 (10.0)1 (0.8)11 (9.2)1 (0.8)
30 - 3412823 (18.0)1 (0.8)9 (7.0)1 (0.8)
35 - 397017 (24.3)1 (1.4)6 (8.6)1 (1.4)
> 40223 (13.6)0 (0.0)4 (18.2)0 (0.0)
Occupation
Artisans7812 (15.3)1 (1.7)11 (14.1)0 (0.0)
Civil servants9413 (13.8)0 (0.0)6 (5.3)1 (1.1)
Farmers162 (12.5)0 (0.0)1 (6.3)0 (0.0)
Teachers455 (11.1)1 (2.2)3 (15.6)0 (0.0)
Traders11524 (20.9)3 (2.6)6 (4.3)2 (1.7)
Unemployed325 (15.6)0 (0.0)5 (9.3)0 (0.0)
Educational status
Primary674 (6.0)1 (1.5)4 (6.0)0 (0.0)
Secondary14516 (11.3)1 (0.7)5 (3.4)1 (0.7)
Tertiary16841 (24.4)3 (1.8)19 (11.3)2 (1.9)
Trimester
First5415 (27.8)1 (1.9)
Second8529 (34.1)3 (3.5)
Third6117 (27.9)1 (1.6)

Abbreviations: IgG, immunoglobulin G; IgM, immunoglobulin M (P = 0.9044); NE, total number examined; NPF, non-pregnant females; PTW, pregnant women.

Table 2. Toxoplasmosis Seroprevalence in Relation to Possible Issues in Port Harcourt
Possibility IssuesNENP IgGNP IgMMean Seperationa
PTWNPFPTWNPF
Engage in farming
Yes23231 (13.4)17 (7.3)3 (1.3)2 (0.9)1.65A
No14830 (20.3)15 (10.1)2 (1.4)1 (0.7)1.35A
Wash fruits
Yes10616 (15.1)10 (9.4)1 (0.9)0 (0.0)0.43B
No27445 (16.4)22 (8.0)4 (1.5)3 (1.1)0.88A
Drink treated Water
Yes632 (3.2)9 (14.3)0 (0.0)3 (4.8)1.12B
No31759 (18.6)23 (7.3)5 (1.6)0 (0.0)1.86A
History of owning pets
Yes11424 (21.1)11 (9.6)2 (1.8)1 (0.9)0.80A
No26637 (13.9)21 (7.9)3 (1.1)2 (0.8)0.70A
Consume suya
Yes417 (17.1)5 (12.2)1 (2.4)0 (0.0)0.65A
No33954 (15.9)27 (8.0)4 (1.2)3 (0.9)1.05A

Abbreviations: IgG, immunoglobulin G; IgM, immunoglobulin M; NE, total number examined; NP, number positive; NPF, non-pregnant females; PTW, pregnant women.

aMeans with different alphabets are statistically significant (P < 0.05).

Figure 1. PCR spectral analysis for samples tested. Legend a, positive control; b, negative tests.

5. Discussion

The overall seroprevalence of 33 % and 19.4% among pregnant women (PTW) and non-pregnant female (NPF) controls, respectively, in Port Harcourt, Nigeria, was observed in this health institution-based study. This report slightly varies with the results obtained in previous studies, which indicated a prevalence of 24.8%, 38.9%, 44.4%, 44.5%, and 64% in Pakistan, South Eastern Iran, Abuja (Nigeria), Northern Tanzania, and Debre-Tabor (Ethiopia), respectively (8, 11-14). The causes of these variations in seroprevalence are attributed to environmental distinctiveness, prevailing risk factors, and hygienic levels (15). Toxoplasmosis in pregnancy can predispose the fetus to serious complications and severe damage once the infection is developed in pregnancy. Therefore, it is recommended that the serum of pregnant women be tested for toxoplasma IgG and IgM to avoid intrauterine infection and complications (11, 12, 15).

The low prevalence of IgM in this study showed that the toxoplasmosis was not recent evidence of seropositivity since the subjects were only significantly positive for IgG Toxoplasma antibodies. This result correlates with other studies, which reported very low insignificant seroprevalence of IgM Toxoplasma antibodies compared to significantly higher IgG Toxoplasma antibodies (11, 12, 16-18)

The samples that were T. gondii-specific IgM-positive were subject to real-time PCR and were all negative for T. gondii infection. This may be due to the fact that peripheral blood was used in the present study, which is likely to have insufficient circulating parasites since IgM antibodies specific for T. gondii may not remain detectable for long periods of time outside the acute infection (1, 7, 19, 20). In this regard, it emphasizes the practicality of other relevant samples (amniotic fluid or placenta tissue) used in the diagnosis of infection due to T. gondii among pregnant women. Although amniotic fluid or placenta samples may be more relevant for the diagnosis of toxoplasmosis by PCR, the cumbersome nature of the procedure is a disadvantage (11, 12).

The findings of the present study highlighted the contests of relating molecular diagnostic tests and serological data and also getting the right samples that are suitable for detection of T. gondii DNA using the PCR method. IgM test that is particular for T. gondii is employed by many laboratories to conclude that a patient had a recent infection (21). Statistically, there was no significant difference in the rate of seropositivity based on age, occupation, educational status, and trimester. This observation is consistent with the findings of previous reports (12, 14, 17). However, a study reported a correlation between acute toxoplasmosis and the first trimester (13).

Factors found to significantly influence the transmission of T. gondii in this study were engagement in farming or gardening, drinking untreated water, and eating unwashed or improperly washed fruits. This observation is largely supported by a large-scale seroepidemiological report on toxoplasmosis conducted in similar studies (21-23). However, some other studies established that raw vegetable consumption was accompanied by a reduced likelihood of T. gondii infection as well as the lack of links between eating raw vegetables and T. gondii infection (11, 17, 24). A possible explanation for our results was the unsanitary eating practices, and food preparation lifestyles among the group studied. More than two-third (83.4%) of the participants of this study did not drink treated water, which increased the likelihood of infection with T. gondii.

5.1. Conclusions

Public health awareness campaigns on toxoplasmosis transmission dynamics and risk factors are advocated. Eating unwashed fruits, drinking untreated water, engaging in farming or gardening without protective gear or equipment should be avoided because they are possible risk factors of Toxoplasma gondii infection. There is a need for the introduction of toxoplasmosis screening among routine antenatal tests in healthcare facilities; this will greatly control congenital toxoplasmosis.

Footnotes

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