Detection and Analysis of Respiratory Pathogens in Hospitalized Children with Acute Respiratory Tract Infections by a Multiplex-PCR Assay Based on the Genetic Analyzer Platform

AUTHORS

Pengbo Guo 1 , Yanhong Wang 1 , Mingjin Zhou 1 , Shiyue Mei 1 , Yibing Cheng 2 , *

1 Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospital, Institute of Pediatric Medicine, Zhengzhou, China

2 Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospital, Pediatric Intensive Care Unit, Zhengzhou, China

How to Cite: Guo P, Wang Y, Zhou M, Mei S, Cheng Y. Detection and Analysis of Respiratory Pathogens in Hospitalized Children with Acute Respiratory Tract Infections by a Multiplex-PCR Assay Based on the Genetic Analyzer Platform, Iran J Pediatr. Online ahead of Print ; 31(2):e110203. doi: 10.5812/ijp.110203.

ARTICLE INFORMATION

Iranian Journal of Pediatrics: 31 (2); e110203
Published Online: April 24, 2021
Article Type: Research Article
Received: October 10, 2020
Revised: February 21, 2021
Accepted: April 1, 2021
Crossmark
Crossmark
CHECKING
READ FULL TEXT

Abstract

Background: Despite the considerable incidence of acute respiratory tract infections (ARTI) among children in Henan province, detailed epidemiological information is limited.

Objectives: Following a retrospective design, the current study aimed to analyze the epidemiological trends of respiratory pathogens in children hospitalized at Henan Children’s Hospital.

Methods: A total of 11306 children (age range, 4 days to 15 years) diagnosed with ARTI admitted to Henan Children’s Hospital from March 1, 2019, to February 29, 2020, were enrolled. Nasopharyngeal swabs, alveolar lavage fluid, or sputum samples were analyzed for the presence of 12 pathogens via a multiplex-PCR assay based on the Genetic Analyzer platform. Data of 11306 samples were eligible for analysis.

Results: The total positive rate was 78.1% (8831/11306). Of 8831 positive samples, 7017 (79.5%, 7017/8831) had a single pathogen and 1814 (20.5%, 1814/11306) had multiple pathogens. Human rhinovirus was the most common pathogen (25.4%, 2874/11306), followed by mycoplasma (18.1%, 2050/11306) and human respiratory syncytial virus (15.8%, 1783/11306). There was no significant difference concerning the positive rate of respiratory pathogens between boys and girls (χ2 = 0.286, P = 0.593). Children were more likely to be infected in autumn and winter than in spring and summer (9722 vs. 1584, respectively).

Conclusions: Human rhinovirus and mycoplasma were the most commonly detected pathogens. The positive rate of chlamydia was independent of the season, while positive rates of other pathogens were season-related. The positive rate of influenza A (H1N1) was independent of age, while for other pathogens, it was age-dependent. This study demonstrated species-level information on the pathogens, which can improve the prevention and treatment of hospitalized children with ARTI.

1. Background

Acute respiratory tract infections (ARTI) are the most common cause of severe disease and mortality in children worldwide, especially in the first year of life (1). Most of these infections are caused by at least one of the following respiratory pathogens, including bacteria like mycoplasma (MP) or chlamydia (CH), viruses, and fungi (2). The identification of respiratory pathogens (RPs) not only paves the way for accurate diagnosis of ARTI but also is associated with the improved effect of therapy, declined hospitalization, and reduced quantity of antibiotics used in the hospital (3).

However, a timely and accurate diagnosis of respiratory pathogens can be challenging. Rapid antigen tests are the common way to detect the influenza virus, but they suffer from deficiencies in sensitivity, with the possibility of false-positive or negative results (2). However, increasing attention is being paid to multiplex-PCR technology, which in addition to its simplicity and lower operation time, can detect multiple RPs in a single sample. In addition, this assay allows detecting co-infections that may cause severe ARTI. The prevalence of ARTI in children varies depending on the region and age (4). However, limited information are available regarding the ARTI in Henan province

2. Objectives

To analyze the pathogens in hospitalized pediatric patients with ARTI in Henan province, we investigated the epidemiology of RPs via multiplex-PCR based on the 3500Dx Genetic Analyzer platform.

3. Methods

3.1. Sample Collection

In the present study, we retrospectively analyzed data of 11306 ARTI patients admitted to Henan Children's Hospital from March 1, 2019, to February 29, 2020. The specimens were transferred from the inpatient department to the laboratory and stored at 4°C for one day. Those younger than 15 years old and patients who were suffering from symptoms of ARTI according to the diagnostic criteria of “Zhu Futang Practical of Pediatrics” (ISBN 978-7-117-19978-O/R·19979) were included in the study. Children with ARTI had at least one of the following symptoms: cough, fever, sore throat, difficulty breathing, nasal congestion, or runny nose. Exclusion criteria were: 1). few cells in the specimen or insufficient amount of nucleic acid; and 2). Incomplete clinical data.

For all children, specimens were collected within 48h of hospitalization. A total of 11306 specimens were collected in this study. Depending on the patient’s status, sputum (n = 523; 4.6%), bronchoalveolar lavage fluid (n = 212; 1.9%) or pharyngeal swab (n = 10571; 93.5%) was obtained by sputum aspirator, bronchoscopy, or pharyngeal swabs (Shenzhen Mairuikelin Technologies Ltd., Shenzhen, China), respectively. Specimens were transferred from the inpatient department to the laboratory and stored at 4 °C at most for one day.

3.2. Total Nucleic Acid Extraction

Nucleic acids were extracted using a throat swab extraction kit (Health Gene Technologies, China)and a Tanbead Nucleic Acid Extractor (Tanwan Advanced Nanotech Inc, China).

3.3. PCR

We used the Respiratory Pathogen Multiplex Detection Kit (Ningbo HEALTH Gene Technologies Ltd., Ningbo, China) for multiplex PCRs, which includes PCR enzyme, dNTPs, MgCl2, primers for each pathogen, and buffer. Multiplex-PCR mixtures contained 14 μL of the pre-mixed solution, 1 μL of enzyme mix (containing uracil-DNA glycosylase enzyme, reverse transcriptase, and DNA polymerase), and 5 μL of the extracted sample. Multiplex PCR was completed on a Verity thermal cycler (Thermo Fisher Scientific Inc) following this methodology: step 1, 25°C for 5 min, 50°C for 15 min, and 95°C for 2 min; step 2, 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s. The second step was iterated for 35 cycles. Then, samples were incubated at 72°C for 10 min and afterward were stored at 4°C. The kit of the multiplex-PCR assay (Health Gene Technologies) can detect 12 pathogens, including human rhinovirus (HRV), human respiratory syncytial virus (HRSV), influenza A H3N2 (H3N2), human adenovirus (HADV), human parainfluenza virus (HPIV), human metapneumovirus (HMPV), influenza B (IFVB), human bocavirus (BOCA), human coronavirus (HCOV), influenza A H1N1(H1N1), CH, and MP.

3.4. Runing on the 3500Dx Genetic Analyzer Platform

Following the multiplex-PCR, 1μL of amplified products and 0.25μL of SIZE-500PLUS (Thermo Fisher Scientific Inc) were added into 8.75μL of Hi-DiTM Formamide (Thermo Fisher Scientific Inc). Then, analysis was performed on the mixed liquid using the 3500DX Genetic Analyzer platform (Thermo Fisher Scientific, USA).

3.5. Statistical Analysis

Raw data were analyzed using SPSS software (version 23.0; IBM Corp., USA). Positive rates among different age groups and seasons were analyzed using the chi-squared test. Statistical significance was considered when P-value < 0.05.

4. Results

4.1. Confirmation of the Assay Based on the 3500DX Genetic Analyzer Platform

Human genomic DNA was used as a negative control, and plasmids carrying the corresponding viral sequences were used as a positive control. The human DNA peak and internal reference were observed in the negative control, while 12 pathogen-specific peaks presented in the positive control, and the peaks separated well. We found no overlap in the positive control (Figure 1), which indicates the accuracy and reliability of the assay based on the 3500DX Genetic Analyzer platform.

Figure 1. Quantitative detection of the assay based on the 3500DX Genetic Analyzer platform. The Y-axis indicates the fluorescent signal, and the X-axis indicates the size of PCR products. A, positive control; B, negative control.

4.2. Characteristics of the Patients

A total of 11306 children with ARTI (6650 boys and 4656 girls) who were hospitalized from March 1, 2019, to February 29, 2020, were included in the present study. The youngest and oldest participants aged 4 days and 15 years, respectively. The general characteristics of participants are provided in Table 1.

Table 1. General Characteristics of Participants
CharacteristicsNo. (%)
Gender
Male6650 (58.8)
Female4656 (41.2)
Age (y)
< 1 2944 (26.0)
1 - 33371 (29.8)
3 - 5 y 2542 (22.5)
5 - 15 y2449 (21.7)
Total11306

4.3. Results of Respiratory Pathogen Detection

The overall positive rate was 78.1% (8831/11306). Of 8831 positive samples, 7017 (79.5%, 7017/8831) had a single pathogen and 1814 (20.5%, 1814/8831) had multiple pathogens. The rate of single infection was 62.5% (7071/11306) and that of multiple infections was 16.0% (1814/11306). HRV was the most common pathogen (25.4%, 2874/11306), followed by MP (18.1%, 2050/11306), and HRSV (15.8%, 1783/11306). The positivity rates of other pathogens were as follows: IFVA-H3N2, 9.0% (1023/11306); HADV, 8.7% (982/11306); HPIV,7.3% (820/11306); HMPV, 3.3% (371/11306); IFVB, 3.2% (367/11306); BOCA, 2.0% (231/11306); HCOV,1.9% (220/11306); Ch,0.4% (46/11306); and IFVA-H1N1, 0.1% (13/11306).

4.4. Age and Gender Distribution of Positive Patients

The positive rates of RPs in boys and girls were 78.5% (5219/6650) and 77.6% (3612/4656), respectively. There was no significant difference between boys and girls concerning the variable of positive rate (χ2 = 0.286, P = 0.593). In this study, using the variable of age, participants were categorized as follows: babies (< 1 year), toddlers (1 - 3 years), preschool children (3 - 5 years), and school children (5 - 15 years). The distribution of RPs in the four groups is shown in Table 2. There was no significant difference among the different groups concerning the total positive rates.

Table 2. Distribution of RPS Separated by the Age
CharacteristicsBabies (< 1y) N = 2944Toddlers (1 - 3y) N = 3371Preschool Children (3 - 5y) N = 2542School Children(5 - 15y) N = 2449All Ages N = 11306Χ2P-Value
No.Pos.Prevalence (%)No.Pos.Prevalence (%)No.Pos.Prevalence (%)No.PosPrevalence (%)No.PosPrevalence (%)
Positive221175.1265378.7207181.5189677.4883178.17.4 > 0.05
Single176359.9203260.3161463.5161065.7701962.110.2 < 0.05
Mixed44815.262118.445718.028611.7181216.048.2 < 0.05
HADV1324.533810.032012.61927.89828.7113.4 < 0.05
BOCA883.01123.3261.050.22312.093.4 < 0.05
HRV81127.594628.169127.242617.4287425.479.7 < 0.05
IFVB491.71113.3953.71134.63673.238.6 < 0.05
CH190.640.160.2170.7460.417.8 < 0.05
H1N120.160.230.120.1130.12.0 > 0.05
MP1143.939611.753421.0100641.1205018.11128.7 < 0.05
H3N21555.32748.130912.228511.610239.095.0 < 0.05
HCOV712.4832.5351.4311.32201.918.0 < 0.05
HRSV85529.061818.328211.1281.1178315.8710.4 < 0.05
HPIV31010.53199.51405.5512.18207.3167.2 < 0.05
HMPV953.21283.81104.3381.63713.333.6 < 0.05

4.5. Seasonal Distribution of RPs

Participants were separated into four groups according to the season: (a) spring (February, March, and April) with 696 cases; (b) summer (May, June, and July) with 888 cases; (c) autumn (August, September, and October) with 3688 cases; and (d) winter (November, December, and January) with 6034 cases. The positive rates of RPs in every season are shown in Table 3. It is worth noting that HRSV was found to be more frequent during the winter, while the positive rate of HRV showed little change throughout the year. Also, MP was more common in autumn. The rest of the RPs had an almost equal incidence throughout the year, i.e., without a significant seasonal trend.

Table 3. Seasonal Distribution of RPs Detected Using the Multiplex-PCR Assay Based on the Genetic Analyzer Platform
Spring (696)Summer (888)Autumn (3688)Winter (6034)Total (11306)Χ2P-Value
No.Pos.Prevalence (%)No.Pos.Prevalence (%)No.Pos.Prevalence (%)No.Pos.Prevalence (%)No.Pos.Prevalence (%)
HADV385.59911.140110.94447.49828.754.0 < 0.05
BOCA40.6424.71052.8801.32312.063.0 < 0.05
HRV547.835439.9145339.4101316.8287425.4561.3 < 0.05
IFVB557.9293.3100.32734.53673.2220.9 < 0.05
CH30.440.5190.5200.3460.44.0 > 0.05
H1N130.400.010.090.1130.112.0 < 0.05
MP15221.811012.486523.592315.3205018.1212.5 < 0.05
H3N2294.2121.4110.397116.110239.0715.0 < 0.05
HCOV20.3404.5962.6821.42201.952.9 < 0.05
HRSV7210.300.0591.6165227.4178315.81157.5 < 0.05
HPIV284.015817.849113.31432.48207.3551.6 < 0.05
HMPV334.710.1130.43245.43713.3232.5 < 0.05

5. Discussion

RPs are the direct cause of ARTI. Hence, their early detection not only can help clinicians to make an accurate diagnosis but also promotes rational prescription of antibiotics. In recent years, there has been an increase in the use of nucleic acid amplification tests (NAAT) for the detection of RPs due to their excellent detection ability (5).

In this study, we found significant differences concerning the distribution of RPs based on the season and age. It is well-proved that the most common pathogen depends on age and season. For instance, a study conducted in Iran reported that the most common respiratory pathogen was HRV in the group younger than 4 years and IFVB in the group of 5 to 50 years (6). Or a study in the United States mentioned BOCA as the most prevalent respiratory pathogen among those younger than 4 years and HRV in the group of 5 to 50 years (7). These reports suggest that the prevalence of RPs depends on the geographic regions, in addition to season and age.

Several studies analyzed the epidemiological patterns of respiratory infections. HRV is usually the most common single pathogen found in ARTI samples, with a prevalence of 24 - 50%, followed by HRSV (22 - 25%), and influenza viruses (7.2 - 8%), either in nasal washes or nasopharyngeal swabs (8-10). In this study, the most common RP was HRV (25.4%, 2874/11306), followed by MP (18.1%, 2050/11306) and HRSV (15.8%, 1783/11306).

According to the findings, gender had no effect on the prevalence positive rate of RPs, which is consistent with the literature (11-13). Many studies concluded that respiratory infections are influenced by the season, with their peaks lasting from winter to early spring (12, 14). In this study, the positive rate of RPs was higher in winter, while it was lower in the spring. Hence, it can be argued that climate has an important role in the prevalence of RPs, since cold air may extend the survival time of RPs in respiratory droplets. Huang et al. reported no significant difference concerning the total RPs positivity rate of different age groups. However, they mentioned that the spectrum of RPs was different depending on the age group (12), which is in line with the findings of the present study. This difference can be attributed to several factors like immunity, guardian status, and range of activity.

The global incidence of influenza ranges from 5 to 10% among adults and 20 to 30% among children (15). Influenza virus can cause severe pneumonia, which may even be fatal. According to the literature, influenza is disproportionate in its effect on different age groups, so that it mainly affects younger children (nearly 870 000 cases of hospitalization among children younger than 5 years) (16, 17). In this study, influenza viruses (IFVA-H1N1 + IFVA-H3N2+IFVB) (1253/6043, 20.73%) were the second most commonly detected RPs in the winter.

In summary, HRV and MP were the most commonly detected pathogens among children with ARTI in Henan province. The positive rate of CH was independent of the season, while it was not true for the positive rate of other pathogens. The positive rate of influenza A (H1N1) was independent from age, while the positive rate of other pathogens was age-related. The multiplex-PCR assay, based on the 3500DX Genetic Analyzer platform, can quickly detect the RPs that are associated with increased risk of ARTI. This ability not only helps clinicians to make an accurate diagnosis but also promotes rational use of antibiotics. This study demonstrated species-level information on the pathogens, which can improve prevention and therapy in hospitalized children with ARTI.

It is necessary to mention some limitations of our study, including following a single-center design, which probably has affected the representativeness of our samples. Also, the study lasted only for one year, which may not fully capture the epidemic trends of RPs.

Footnotes

References

  • 1.

    Panda S, Mohakud NK, Pena L, Kumar S. Human metapneumovirus: review of an important respiratory pathogen. Int J Infect Dis. 2014;25:45-52. doi: 10.1016/j.ijid.2014.03.1394. [PubMed: 24841931]. [PubMed Central: PMC7110553].

  • 2.

    Lin CY, Hwang D, Chiu NC, Weng LC, Liu HF, Mu JJ, et al. Increased detection of viruses in children with respiratory tract infection using PCR. Int J Environ Res Public Health. 2020;17(2). doi: 10.3390/ijerph17020564. [PubMed: 31952364]. [PubMed Central: PMC7013517].

  • 3.

    Liu GS, Li H, Zhao SC, Lu RJ, Niu PH, Tan WJ. Viral and bacterial etiology of acute febrile respiratory syndrome among patients in Qinghai, China. Biomed Environ Sci. 2019;32(6):438-45. doi: 10.3967/bes2019.058. [PubMed: 31262389]. [PubMed Central: PMC7135780].

  • 4.

    Wen X, Huang Q, Tao H, Zou W, Gao M, Guo H, et al. Clinical characteristics and viral etiologies of outpatients with acute respiratory infections in Huzhou of China: a retrospective study. BMC Infect Dis. 2019;19(1):32. doi: 10.1186/s12879-018-3668-6. [PubMed: 30621623]. [PubMed Central: PMC6325799].

  • 5.

    Qian Y, Ai J, Wu J, Yu S, Cui P, Gao Y, et al. Rapid detection of respiratory organisms with FilmArray respiratory panel and its impact on clinical decisions in Shanghai, China, 2016-2018. Influenza Other Respir Viruses. 2020;14(2):142-9. doi: 10.1111/irv.12701. [PubMed: 31786832]. [PubMed Central: PMC7040966].

  • 6.

    Javadi A, Adibi P, Ataei B, Nokhodian Z, Yaran M. Surveillance of acute respiratory infections among outpatients: A pilot study in Isfahan city. J Res Med Sci. 2015;20(2):115-21. [PubMed: 25983761]. [PubMed Central: PMC4400703].

  • 7.

    Goktas S, Sirin MC. Prevalence and seasonal distribution of respiratory viruses during the 2014 - 2015 season in Istanbul. Jundishapur J Microbiol. 2016;9(9):39132. doi: 10.5812/jjm.39132. [PubMed: 27800148]. [PubMed Central: PMC5086027].

  • 8.

    Feikin DR, Njenga MK, Bigogo G, Aura B, Aol G, Audi A, et al. Viral and bacterial causes of severe acute respiratory illness among children aged less than 5 years in a high malaria prevalence area of western Kenya, 2007-2010. Pediatr Infect Dis J. 2013;32(1):e14-9. doi: 10.1097/INF.0b013e31826fd39b. [PubMed: 22914561].

  • 9.

    Yu X, Kou Y, Xia D, Li J, Yang X, Zhou Y, et al. Human respiratory syncytial virus in children with lower respiratory tract infections or influenza-like illness and its co-infection characteristics with viruses and atypical bacteria in Hangzhou, China. J Clin Virol. 2015;69:1-6. doi: 10.1016/j.jcv.2015.05.015. [PubMed: 26209367]. [PubMed Central: PMC7185398].

  • 10.

    Lau YF, Koh WV, Kan C, Dua PA, Lim AE, Liaw CJ, et al. Epidemiologic analysis of respiratory viral infections among Singapore military servicemen in 2016. BMC Infect Dis. 2018;18(1):123. doi: 10.1186/s12879-018-3040-x. [PubMed: 29529993]. [PubMed Central: PMC5848554].

  • 11.

    Kurskaya O, Ryabichenko T, Leonova N, Shi W, Bi H, Sharshov K, et al. Viral etiology of acute respiratory infections in hospitalized children in Novosibirsk City, Russia (2013 - 2017). PLoS One. 2018;13(9). e0200117. doi: 10.1371/journal.pone.0200117. [PubMed: 30226876]. [PubMed Central: PMC6143185].

  • 12.

    Li YT, Liang Y, Ling YS, Duan MQ, Pan L, Chen ZG. The spectrum of viral pathogens in children with severe acute lower respiratory tract infection: A 3-year prospective study in the pediatric intensive care unit. J Med Virol. 2019;91(9):1633-42. doi: 10.1002/jmv.25502. [PubMed: 31081548]. [PubMed Central: PMC7167151].

  • 13.

    Esposito S, Mencacci A, Cenci E, Camilloni B, Silvestri E, Principi N. Multiplex platforms for the identification of respiratory pathogens: Are they useful in pediatric clinical practice? Front Cell Infect Microbiol. 2019;9:196. doi: 10.3389/fcimb.2019.00196. [PubMed: 31275863]. [PubMed Central: PMC6593267].

  • 14.

    Chen J, Hu P, Zhou T, Zheng T, Zhou L, Jiang C, et al. Epidemiology and clinical characteristics of acute respiratory tract infections among hospitalized infants and young children in Chengdu, West China, 2009-2014. BMC Pediatr. 2018;18(1):216. doi: 10.1186/s12887-018-1203-y. [PubMed: 29976175]. [PubMed Central: PMC6034247].

  • 15.

    Huang H, Chen S, Zhang X, Hong L, Zeng Y, Wu B. Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP-based multiplex-PCR assay. J Clin Lab Anal. 2020;34(4). e23127. doi: 10.1002/jcla.23127. [PubMed: 31774213]. [PubMed Central: PMC7147121].

  • 16.

    El Guerche-Seblain C, Moureau A, Schiffler C, Dupuy M, Pepin S, Samson SI, et al. Epidemiology and burden of influenza in healthy children aged 6 to 35 months: analysis of data from the placebo arm of a phase III efficacy trial. BMC Infect Dis. 2019;19(1):308. doi: 10.1186/s12879-019-3920-8. [PubMed: 30947693]. [PubMed Central: PMC6449994].

  • 17.

    Lafond KE, Nair H, Rasooly MH, Valente F, Booy R, Rahman M, et al. Correction: Global role and burden of influenza in pediatric respiratory hospitalizations, 1982-2012: A systematic analysis. PLoS Med. 2016;13(6):1002060. doi: 10.1371/journal.pmed.1001977. [PubMed: 27275597].

  • Copyright © 2021, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
    COMMENTS

    LEAVE A COMMENT HERE: