Molecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran


Akram Najafi 1 , Maryam Zare 1 , Mohammad Kargar 1 , *

1 Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

How to Cite: Najafi A, Zare M, Kargar M. Molecular Epidemiology of Rotavirus Strains Circulating among Children with Gastroenteritis in Iran, Iran J Pediatr. 2016 ; 22(1):63-69.


Iranian Journal of Pediatrics: 22 (1); 63-69
Published Online: March 31, 2012
Article Type: Research Article
Received: December 24, 2010
Accepted: September 25, 2011


Objective: This study was conducted to evaluate the prevalence of rotavirus disease and to investigate the genotypes of rotavirus strains causing acute gastroenteritis among children aged <5 years old in Marvdasht, Iran.
Methods: One hundred and forty-one children, aged 1 month to 5 years, afflicted with severe diarrhea were enrolled during January 2007 to December 2008. Their stool samples were studied with enzyme immunoassays (EIA) for group A rotaviruses. Rotavirus-positive specimens were genotyped by the Nested RT-PCR using different types of specific primers.
Findings: Out of total collected samples rotavirus infection was detected in 40 (28.37%). Of the rotavirus episodes, 72.91% occurred during the first 2 years of life (P=0.038). The highest prevalence of infection was identified in summer (52.50%) and the lowest in winter (7.50%). The most common clinical features included diarrhea (96.25%), vomiting (82.50%) and fever (45.0%). Mixed genotypes were the predominant G type (60.0%), followed by non-typeable (12.50%), G2 (12.50%), G4 (10.0%) and G1 (5.0%) genotypes. G3/G8 mixed infection is the first of these rotavirus genotypes to be reported in Iran.
Conclusion: Regarding high frequency of rotavirus infection, continuous surveillance is needed to inform diarrhea prevention programs as well as to provide information about the occurrence of new rotavirus strains. This will assist policy makers in decision making on rotavirus vaccine introduction.




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