Effects of Melatonin on Neurological Function and Maintenance of Physical and Motor Fitness in Collegiate Student-Athletes Following Sleep Deprivation


Nesa Paryab 1 , Morteza Taheri 1 , Kahdijeh Irandoust 1 , Masoud Mirmoezzi ORCID 2 , *

1 Imam Khomeini International University, Qazvin, Iran

2 Faculty of Physical Education and Sport Science, Islamic Azad University, Tehran, Iran

How to Cite: Paryab N , Taheri M, Irandoust K , Mirmoezzi M. Effects of Melatonin on Neurological Function and Maintenance of Physical and Motor Fitness in Collegiate Student-Athletes Following Sleep Deprivation, Int J Sport Stud Hlth. Online ahead of Print ; 3(2):e110657. doi: 10.5812/intjsh.110657.


International Journal of Sport Studies for Health: 3 (2); e110657
Published Online: November 21, 2020
Article Type: Research Article
Received: October 26, 2020
Accepted: November 9, 2020


Background: Melatonin is one of the Supplements used to treat sleep problems such as insomnia and jet lag.

Objectives: Since sleep deprivation may affect athletic performance, the aim of this study was to investigate the effect of melatonin on neurological function and maintenance of physical and motor fitness in collegiate student-athletes following sleep deprivation.

Methods: Ten collegiate student-athletes participated in randomized, double‐blind crossover trial with placebo control. Subjects were divided into six experimental groups: without sleep deprivation (WSD), 4 hours sleep deprivation (4HSD) and 24 hours sleep deprivation (24HSD) with melatonin (MEL) or placebo (PLA). WSD were allowed to sleep eight hours per night. Six milligrams of melatonin was administered 30 min before the training protocols. Training protocols included the Wingate Anaerobic test, Good Balance test, Vienna reaction time with the Stroop test. Data were analyzed using repeated measures ANOVA. Significant difference was set at P ≤ 0.05.

Results: Six mg/day of MEL 30 min before training had no significant effect on anaerobic power, balance and reaction time in collegiate student-athletes WSD (P > 0.05). Although, 4HSD and 24HSD negatively affected balance function, MEL reduced its negative effects. Furthermore, 24HSD decrease neurological and physical performance in collegiate student-athletes and MEL improved anaerobic power and reaction time in collegiate student-athletes (P < 0.05).

Conclusions: Pre-training MEL supplementation would alleviate neurological, physical and motor performance impairment in collegiate student-athletes following sleep deprivation. MEL appears to be more effective in athletes with longer sleep deprivation.

1. Background

Sleep plays a vital role in the body’s biological functions and sleep deprivation can have significant effects on athletes’ performance (1). Athletes and coaches also believe that getting enough sleep is essential to achieving peak performance, but conditions such as long trips and pre-competition anxiety would probably provide the athletes with sleep deprivation. Nevertheless, sleep function is not fully understood and few studies have focused on the effects of sleep deprivation on neurological training performance (2). On the other hand, insomnia is common among collegiate students due to the heavy academic performance and socio-cultural requirements of living in dormitories (3).

The existing literature indicates that lack of sleep can affect motor psychomotor function including simple and selective reaction time (4); it can also have a negative effect on physical function (3) and since special neurological and physical function are important factors on athletic performance, it can change the outcome of the game (4).

While, sleep problems are a potential risk factor for injuries, it has been shown that 24HSD would lead to injury in athletes due to physical and cognitive impairment (5).

Melatonin helps regulate circadian rhythm. The circadian rhythm of MEL release from the pineal gland is highly consistent with the hours of sleep habits and helps regulate circadian rhythm and synchronize our sleep-wake cycle with night and day. MEL responds to changes in light intensity, and the secretion of this hormone reaches a minimum in the morning and a maximum (10 times in the morning) at night. Since melatonin affects body temperature regulation and circadian rhythms, few studies have considered the relationship between MEL and neurological and athletic function (6).

Previous studies have shown that daily consumption of MEL had no significant effect on cognitive and physical function in the morning and one-hour before the start of physical activity in soccer players (1, 7). On the other hand, research emphasizes on improving neurological and physical-motor function following MEL consumption (6, 8).

2. Objectives

In brief, the results of research on the effect of MEL on athletic performance are very limited and the results are contradictory. On the other hand, previous studies used doses lower than 6 mg and the results showed no significant difference (6). Considering the importance of sleep in athletes, we examined the acute effect of MEL following long-term and partial sleep deprivation on neurological function (reaction time) and some indicators of physical and motor fitness (anaerobic power, static and dynamic balance).

3. Methods

The present study was a double-blind randomized placebo-controlled trial. Ten collegiate student-athletes volunteered from of Imam Khomeini International University (Male 18 - 25 y with body mass index (BMI) between 18 - 25 kg/m2, high score 5 in sleep quality test and no difference in micronutrient and macronutrient intake). Inclusion criteria include: (A) History of the championship; (B) no history of acute or chronic illness; (C) no medication or dietary supplement in the previous 6 months; (D) no smoking and alcohol use; and (E) depression Less than 13 according to Beck Depression inventory. Exclusion criteria were (A) having vigorous physical activity 48 hours before or during the study; (B) disturbing the circadian rhythm before the protocol; and (C) unwillingness to continue participating in the study. The research protocol was approved by the Research Ethics Committee of Imam Khomeini University with the code IR.QUMS.REC.1397.402.

Six groups with crossover method set in three sleep conditions of 24HSD, 4HSD and WSD and two supplementation conditions (six Mg/day MEL was taken 30 min pre of training protocols at 5 AM and placebo). One week before the study, the volunteers were given a special diet. 72 hours were allocated for recovery of individuals after the training protocols.

In this study, the Vienna Functional test was used to assess short-term attention and concentration before and after the interventions. Short-term attention and concentration assessment test is one of the neurological tests to examine cognitive and mental components such as reaction time, selective accuracy, attention and concentration, the overall result of which shows the subject’s selective attention capacity (1). The Wingate cycle ergometer (model 894E, Monark, Sweden) was used to measure anaerobic power. Subjects began to pedal for 5 min (loading one watt/kg of body weight) and then the main protocol was performed as a 30-second repetition with 7.5% of body weight. Finally, the anaerobic power output index (peak, relative and average) was recorded as the anaerobic power index (9). The Good balance instrument (Metitur, Jyvaskyla, Finland) was used to measure static and dynamic balance. The feedback provided by the visual stimuli was given to the subjects to maintain balance in both static and dynamic positions. The validity and reliability of this tool have been confirmed in previous studies (10).

The normal distribution of data was confirmed using the Shapiro-Wilk test. The Mauchly test was tested and confirmed to be spherical. Statistical analysis was performed using SPSS software (SPSS Inc., Chicago, IL). Repeated analysis of variance was performed to analyze the data with a significant of less than 0.05.

4. Results

Table 1 shows Neurological and physical performance tests for six experimental conditions [3 sleep conditions (WSD, 4HSD, 24HSD) × 2 supplementation conditions (MEL and PLA)]. The results of repeated measure ANOVA indicated significant differences among the means of groups; each group was compared by the Bonferroni’s method for post hoc test (Table 1).

Table 1. Neurological and Physical Performance Tests for Six Experimental Conditionsa
Reaction timeRC, sMEL0.88 ± 0.040.85 ± 0.040.82 ± 0.07c, d, e0.001h
PLA0.87 ± 0.030.86 ± 0.040.8 ± 0.06f, g
RI, sMEL1.18 ± 0.081.15 ± 0.051.12 ± 0.08c, d, e0.001h
PLA1.19 ± 0.071.15 ± 0.041.1 ± 0.07f, g
NC, sMEL0.87 ± 0.040.82 ± 0.030.79 ± 0.03c, d, e0.001h
PLA0.88 ± 0.030.84 ± 0.030.75 ± 0.02f, g
NI, sMEL0.85 ± 0.040.84 ± 0.030.83 ± 0.03c, d, e0.003h
PLA0.85 ± 0.030.84 ± 0.020.83 ± 0.02f, g
Anaerobic powerRPP, W/kgMEL11.09 ± 1.810.76 ± 1.910.48 ± 1.6c, d, e0.026h
PLA11.01 ± 1.710.88 ± 1.710.03 ± 1.5f, g
RAP, W/kgMEL8.12 ± 1.38.02 ± 1.27.48 ± 1c, d, e0.015h
PLA8.15 ± 1.47.97 ± 1.27.54 ± 1.1f, g
RMP, W/kgMEL5.15 ± 1.44.69 ± 1.41.32 ± 1.2c, d, e0.000h
PLA5.18 ± 1.44.63 ± 1.34.11 ± 1.3f, g
Static balanceSpeedx, mm/sMEL0.35 ± 0.20.37 ± 0.2g0.44 ± 0.1c, d0.000h
PLA0.36 ± 0.20.4 ± 0.20.49 ± 0.1f
Speedy, mm/sMEL0.71 ± 0.20.73 ± 0.1g0.8 ± 0.2c, d0.003h
PLA0.72 ± 0.10.75 ± 0.20.88 ± 0.2f
Dynamic balanceSpeedtrav, mm/sMEL130 ± 75.6112.4 ± 41.2f, g100.1 ± 40.7c, d, e0.000h
PLA128.1 ± 74.9114.7 ± 47.7f109.7 ± 44.7f

Abbreviations: MEL: Melatonin, PLA: Placebo.

aValues are expressed as mean ± SD.

bP value for repeated-measures ANOVA.

cSignificant difference with 24HSD PLA.

dSignificant difference with WSD MEL.

eSignificant difference with 4HSD MEL.

fSignificant difference with WSD PLA.

gSignificant difference with 4HSD PLA.

hIndicates significant level at 0.05.

As shown in Table 1, 6 mg/day MEL ingestion has not significant effect (P > 0.05) on neurological and physical performance in collegiate student-athletes during the without a sleep deprivation night.

The results of repeated measure ANOVA showed a significant difference in the means of 24HSD (F(5, 54), P < 0.05). In reaction time and anaerobic power, 6 mg/day MEL ingestion during 24HSD has a significant improvement compare with 24HSD with PLA and is significantly lower than 6 mg/day MEL ingestion during WSD/4HSD (P < 0.05). PLA ingestion during 24HSD is significantly lower than WSD/4HSD with PLA (P < 0.05). In static balance, 6 mg/day MEL ingestion during 24HSD has a significant improvement compare with 24HSD with PLA and is significantly lower than WSD with ingestion PLA (P < 0.05). PLA ingestion during 24HSD is significantly lower than WSD with PLA (P < 0.05).

In dynamic balance, 6 mg/day MEL ingestion during 24HSD has a significant improvement compare with 24HSD with PLA and is significantly lower than WSD/4HSD with ingestion MEL (P < 0.05). PLA ingestion during 24HSD is significantly lower than WSD with PLA (P < 0.05).

5. Discussion

The results of the present study showed that taking 6 mg/day of MEL has no effect on improving reaction time, anaerobic power and balance of collegiate student-athletes without sleep deprivation. Also, taking six mg/day of MEL 30 min before training would improve the static and dynamic balance of collegiate student-athletes with 4HSD. Finally, it was shown that 24HSD led to a decrease in neurological function and some physical factors in collegiate student-athletes, however, taking 6 mg/day of MEL 30 min before training significantly reduced the negative effects of sleep deprivation.

Previous research has shown that recovery process occurred three to four times faster in adolescents with sleep disorders who take MEL use improved 67% of patients with sleep disorders (11). Laboratory research has shown that a number of sensory, perceptual, and motor functions can be altered by insomnia or lack of adequate sleep. The circadian rhythm of sleep has positive effects on improving the athletic performance and if this rhythm is disturbed for any reason, it has negative effects on the quality of athletes’ performance (12). Insomnia has a great effect on cognitive impairment, accuracy and concentration and even the quality of training (13).

The researchers stated that short-term sleep deprivation has no effect on anaerobic function, while it has a negative effect on cognitive function such as reaction time (1, 14). However, other studies have shown that partial sleep deprivation leads to a decrease in mean and peak anaerobic power (9, 15). One of the possible mechanisms of positive effects of MEL on anaerobic capacity is the increase of liver lactate concentration and decreasing plasma lactate concentration after activity. MEL also increases intracellular calcium in muscles and liver, which helps to improve the muscle contraction mechanism in anaerobic activity (16).

MEL has a positive effect on improving equilibrium and balance with its effects on the cerebellum. Reduced heart rate and better physiological responses also lead to visual acuity, sensory mechanisms, atrial system, postural control, and balance. Research has also shown that the negative effects of sleep deprivation on postural control would be due to reduced visual input, atrial system function, and impaired musculoskeletal function (17). Research has shown that partial sleep deprivation increases reaction time and decreases selective and continuous attention (1). Research has shown that performance on the Stroop test is highly associated with an increase in reaction time and the number of errors following sleep deprivation in females (12). Sleep deprivation affects the entire attention network, which is made up of many closely related connections to cortical areas. It also increases the level of stress hormones and reduces constant attention and functional memory (18). It should be noted that the frontal lobe is highly sensitive to sleep deprivation which reduces brain activity and also a decrease in neurological function occurs through the thalamus and peripheral cortex by which both parts mediate the network of consciousness, attention, and cognitive processes (1, 18). Anxiety, psychological and physical stress following sleep deprivation appears to weaken the body’s response to sudden stimuli. MEL consumption can affect neuromuscular accuracy and coordination by increasing perceptual and cognitive activity of the brain and concentration of the senses through sympathetic inhibition (6, 7).

There are some limitations in the study. The sample size was small. On the other hand, although subjects were instructed to maintain their usual diet and activity levels, no exact monitoring was performed; the experiment was also performed in dormitory conditions and it was not possible to control the level of stress in all subjects.

5.1. Conclusions

MEL has no effect on the neurological or physical function of athletes who are not deprived of sleep. However, during 4HSD, when the accuracy of postural control decreases, and in 24HSD, as reaction time, anaerobic power and balance are negatively affected, taking 6 mg/day of MEL 30 min before training greatly reduces the negative effects of sleep deprivation.




  • 1.

    Taheri M, Arabameri E. The effect of sleep deprivation on choice reaction time and anaerobic power of college student athletes. Asian J Sports Med. 2012;3(1):15-20. doi: 10.5812/asjsm.34719. [PubMed: 22461961]. [PubMed Central: PMC3307962].

  • 2.

    Halson SL. Sleep in elite athletes and nutritional interventions to enhance sleep. Sports Med. 2014;44 Suppl 1:S13-23. doi: 10.1007/s40279-014-0147-0. [PubMed: 24791913]. [PubMed Central: PMC4008810].

  • 3.

    Patrick Y, Lee A, Raha O, Pillai K, Gupta S, Sethi S, et al. Effects of sleep deprivation on cognitive and physical performance in university students. Sleep Biol Rhythms. 2017;15(3):217-25. doi: 10.1007/s41105-017-0099-5. [PubMed: 28680341]. [PubMed Central: PMC5489575].

  • 4.

    Monleon C, Hemmati Afif A, Mahdavi S, Rezayi M. The Acute Effect of Low Intensity Aerobic Exercise on Psychomotor Performance of Athletes with Nocturnal Sleep Deprivation. Int J Sport Stud Health. 2018;1(1). e66783. doi: 10.5812/intjssh.66783.

  • 5.

    Gao B, Dwivedi S, Milewski MD, Cruz AJ. Lack of Sleep and Sports Injuries in Adolescents: A Systematic Review and Meta-analysis. J Pediatr Orthop. 2019;39(5):e324-33. doi: 10.1097/BPO.0000000000001306. [PubMed: 30888337].

  • 6.

    López-Flores M, Luque-Nieto R, Moreira OC, Suárez-Iglesias D, Villa-Vicente JG. Effects of melatonin on sports performance: A systematic review. JEP Online. 2018;21:121-38.

  • 7.

    Ghattassi K, Hammouda O, Graja A, Boudhina N, Chtourou H, Hadhri S, et al. Morning melatonin ingestion and diurnal variation of short-term maximal performances in soccer players. Physiol Int. 2016;103(1):94-104. doi: 10.1556/036.103.2016.1.9. [PubMed: 27030631].

  • 8.

    Leonardo-Mendonca RC, Martinez-Nicolas A, de Teresa Galvan C, Ocana-Wilhelmi J, Rusanova I, Guerra-Hernandez E, et al. The benefits of four weeks of melatonin treatment on circadian patterns in resistance-trained athletes. Chronobiol Int. 2015;32(8):1125-34. doi: 10.3109/07420528.2015.1069830. [PubMed: 26361788].

  • 9.

    Harbili S. The Effect of Different Recovery Duration on Repeated Anaerobic Performance in Elite Cyclists. J Hum Kinet. 2015;49:171-8. doi: 10.1515/hukin-2015-0119. [PubMed: 26839617]. [PubMed Central: PMC4723166].

  • 10.

    Mansfield A, Aqui A, Fraser JE, Rajachandrakumar R, Lakhani B, Patterson KK. Can augmented feedback facilitate learning a reactive balance task among older adults? Exp Brain Res. 2017;235(1):293-304. doi: 10.1007/s00221-016-4790-6. [PubMed: 27709269]. [PubMed Central: PMC5233445].

  • 11.

    Bramley H, Henson A, Lewis MM, Kong L, Stetter C, Silvis M. Sleep Disturbance Following Concussion Is a Risk Factor for a Prolonged Recovery. Clin Pediatr (Phila). 2017;56(14):1280-5. doi: 10.1177/0009922816681603. [PubMed: 29073787].

  • 12.

    Thun E, Bjorvatn B, Flo E, Harris A, Pallesen S. Sleep, circadian rhythms, and athletic performance. Sleep Med Rev. 2015;23:1-9. doi: 10.1016/j.smrv.2014.11.003. [PubMed: 25645125].

  • 13.

    Eagles AN, Lovell DI. Changes in sleep quantity and efficiency in professional rugby union players during home-based training and match play. J Sports Med Phys Fitness. 2016;56(5):565-71. [PubMed: 27285344].

  • 14.

    Taheri M, Irandost K, Mirmoezzi M, Ramshini M. Effect of Aerobic Exercise and Omega-3 Supplementation on psychological aspects and Sleep Quality in Prediabetes Elderly Women. Sleep Hypn Int J. 2018:170-4. doi: 10.5350/Sleep.Hypn.2019.21.0185.

  • 15.

    Abedelmalek S, Chtourou H, Aloui A, Aouichaoui C, Souissi N, Tabka Z. Effect of time of day and partial sleep deprivation on plasma concentrations of IL-6 during a short-term maximal performance. Eur J Appl Physiol. 2013;113(1):241-8. doi: 10.1007/s00421-012-2432-7. [PubMed: 22677919].

  • 16.

    Beck WR, Scariot PP, Gobatto CA. Melatonin is an Ergogenic Aid for Exhaustive Aerobic Exercise only during the Wakefulness Period. Int J Sports Med. 2016;37(1):71-6. doi: 10.1055/s-0035-1559698. [PubMed: 26509365].

  • 17.

    Kamoun A, Hammouda O, Yahia A, Dhari O, Ksentini H, Driss T, et al. Effects of Melatonin Ingestion Before Nocturnal Sleep on Postural Balance and Subjective Sleep Quality in Older Adults. J Aging Phys Act. 2019;27(3):316-24. doi: 10.1123/japa.2018-0107. [PubMed: 30160577].

  • 18.

    Stenuit P, Kerkhofs M. Effects of sleep restriction on cognition in women. Biol Psychol. 2008;77(1):81-8. doi: 10.1016/j.biopsycho.2007.09.011. [PubMed: 18006139].

  • Copyright © 2020, International Journal of Sport Studies for Health. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.