Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp


Ebrahim Khodaverdi Darian 1 , Mohammad Mahdi Forghanifard 2 , Soheila Moradi 3 , Yung-Fu Chang 4 , emad yahaghi 5 , Majid esmaelizad 6 , Maryam Khaleghizadeh 2 , Pejvak Khaki 3 , *

1 Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, IR Iran

2 Department of Biology, Damghan Branch, Islamic Azad University, Damghan, IR Iran

3 Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, IR Iran

4 Department of Population, Medicine and Diagnostic sciences, College of Veterinary Medicine, Cornell University, Ithaca, USA

5 Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran

6 Department of Biotechnology, Razi Vaccin and Serum Resaerch Institute, Karaj, IR Iran

How to Cite: Khodaverdi Darian E, Forghanifard M M, Moradi S, Chang Y, yahaghi E, et al. Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp, Iran Red Crescent Med J. 2013 ; 15(11):e95982. doi: 10.5812/ircmj.8793.


Iranian Red Crescent Medical Journal: 15 (11); e95982
Published Online: November 05, 2013
Article Type: Research Article
Received: July 03, 2019
Accepted: August 03, 2013


Background: Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira species. A major challenge of this disease is the application of basic research to improve diagnostic methods and related vaccine development. Outer membrane proteins of Leptospira are potential candidates that may be useful as diagnostic or immunogenic factors in treatment and analysis of the disease.

Objectives: To develop an effective subunit vaccine against prevalent pathogenic Leptospira species, we sequenced and analyzed the LipL32 gene from three different Leptospira interrogans (L.interrogans) vaccinal serovars in Iran.

Materials and Methods: Following DNA extraction from these three serovars, the related LipL32 genes were amplified and cloned in the pTZ57R/T vector. Recombinant clones were confirmed by colony- PCR and DNA sequencing. The related sequences were subjected to homology analysis by comparing them to sequences in the Genbank database.

Results: The LipL32 sequences were >94% homologous among the vaccinal and other pathogenic Leptospira serovars in GenBank. This result indicates the conservation of this gene within the pathogenic Leptospires.

Conclusions: The cloned gene in this study may provide a potentially suitable platform for development of a variety of applications such as serological diagnostic tests or recombinant vaccines against leptospirosis.


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