Tuberculosis (TB) remains a worldwide healthcare concern and has been characterized as an epidemic by World Health Organization (WHO). In the last few decades, DNA fingerprint techniques have become available to study the transmission of TB and other mycobacterial infections. The main gold standard typing technique is restriction fragment length polymorphism, which is widely used for molecular epidemiology purposes. However, other recently introduced techniques such as spoligotyping and MIRU-VNTR are also widely used. This review briefly summarizes the recent achievements in TB epidemiology associated with the introduction of molecular typing methods for Mycobacterium tuberculosis complex and non-M. tuberculosis complex mycobacteria. Besides, in this article, the application of molecular methods in the study of epidemiological aspects and diagnosis of mycobacterial diseases are highlighted.

"/> Tuberculosis (TB) remains a worldwide healthcare concern and has been characterized as an epidemic by World Health Organization (WHO). In the last few decades, DNA fingerprint techniques have become available to study the transmission of TB and other mycobacterial infections. The main gold standard typing technique is restriction fragment length polymorphism, which is widely used for molecular epidemiology purposes. However, other recently introduced techniques such as spoligotyping and MIRU-VNTR are also widely used. This review briefly summarizes the recent achievements in TB epidemiology associated with the introduction of molecular typing methods for Mycobacterium tuberculosis complex and non-M. tuberculosis complex mycobacteria. Besides, in this article, the application of molecular methods in the study of epidemiological aspects and diagnosis of mycobacterial diseases are highlighted.

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Genotyping and identification of mycobacteria by fingerprinting techniques

AUTHORS

Azar Dokht Khosravi 1 , * , Sepideh Seghatoleslami 2

1 Department of Medical Microbiology, School of Medicine, and Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, [email protected], Iran

2 Department of Medical Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Iran

How to Cite: Khosravi A D, Seghatoleslami S. Genotyping and identification of mycobacteria by fingerprinting techniques, Jundishapur J Microbiol. Online ahead of Print ; 2(3):81-91.

ARTICLE INFORMATION

Jundishapur Journal of Microbiology: 2 (3); 81-91
Article Type: Research Article
Received: July 1, 2009
Accepted: September 1, 2009
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Abstract

Tuberculosis (TB) remains a worldwide healthcare concern and has been characterized as an epidemic by World Health Organization (WHO). In the last few decades, DNA fingerprint techniques have become available to study the transmission of TB and other mycobacterial infections. The main gold standard typing technique is restriction fragment length polymorphism, which is widely used for molecular epidemiology purposes. However, other recently introduced techniques such as spoligotyping and MIRU-VNTR are also widely used. This review briefly summarizes the recent achievements in TB epidemiology associated with the introduction of molecular typing methods for Mycobacterium tuberculosis complex and non-M. tuberculosis complex mycobacteria. Besides, in this article, the application of molecular methods in the study of epidemiological aspects and diagnosis of mycobacterial diseases are highlighted.

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