Investigation the Effect of Encapsulated Bromelain Enzyme in Magnetic Carbon Nanotubes on Colorectal Cancer Cells


Ardalan Montazeri ORCID 1 , Mina Ramezani ORCID 1 , * , Azadeh Mohammadgholi ORCID 1

1 Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran

How to Cite: Montazeri A, Ramezani M, Mohammadgholi A. Investigation the Effect of Encapsulated Bromelain Enzyme in Magnetic Carbon Nanotubes on Colorectal Cancer Cells, Jundishapur J Nat Pharm Prod.2021 In Press(In Press): e108796.


Jundishapur Journal of Natural Pharmaceutical Products: In Press (In Press); e108796
Published Online: May 30, 2021
Article Type: Research Article
Received: August 24, 2020
Revised: December 19, 2020
Accepted: December 23, 2020
Accepted Article scheduled for 16 (3)


Background: Bromelain (BL) is an enzyme extracted from Ananas comosus plant, which has been known for the therapeutic properties.

Objectives: The anticarcinogenic activity of BL enzyme was examined with and without the presence of Magnetic Carbon Nanotubes (MCNTs) against HT-29 colorectal cancer cells.

Methods: The operational factors affecting BL adsorption such as contact time (30, 60, 90, 120 and 180 min), adsorbent dosage (1 g/L, 5 g/L), initial bromelain concentration (50, 150 and 300 mg/L) and temperature (35 and 50 °C) were studied in details. Then cancer cells were exposed to various BL concentrations (0.1 μg/mL, 1 μg/mL, 10 μg/mL, and 100 μg/mL) and the cell viability was determined by Methylthiazol Tetrazolium (MTT) Assay after 24, 48 and 72 h.

Results: The highest adsorption of BL on nanotubes was 41.62 mg/L and achieved at 35 °C and 90 min with 50 mg/L initial BL concentration and 1g/L MCNTs. The adsorption followed the Freundlich model and second-order kinetics. The results indicated that MCNTs could be a potential effective adsorbent for the removal of BL.

Conclusions: MTT assay indicated that a 100 μg/mL concentration of BL alone and in combination with MCNTs efficiently inhibited the HT-29 cancerous cells. However, encapsulated BL had a considerable advantage of slow delivery which is favorable for cancer treatment.


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