The Effects of Different Media on Shoot Proliferation From the Shoot Tip of Aloe vera L.


Mohammad Hosein Daneshvar 1 , * , Noorolah Moallemi 2 , Nazanin Abdolah Zadeh 2

1 Department of Horticulture, Faculty of Agriculture, Ramin Agricultural and Natural Resources University, IR Iran

2 Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz , IR Iran

How to Cite: Daneshvar M H, Moallemi N, Abdolah Zadeh N. The Effects of Different Media on Shoot Proliferation From the Shoot Tip of Aloe vera L., Jundishapur J Nat Pharm Prod. Online ahead of Print ; 8(2):93-97. doi: 10.17795/jjnpp-4820.


Jundishapur Journal of Natural Pharmaceutical Products: 8 (2); 93-97
Published Online: May 4, 2013
Article Type: Research Article
Received: March 10, 2012
Accepted: December 23, 2012


Background: Aloe vera L. is an important pharmaceutical plant from which several medicinal and cosmetic compounds are extracted. Aloe is naturally propagated through offset, which is a slow and expensive labor cost method with low economical income.

Objectives: In this study, the effect of different media on shoot proliferation of the shoot tip of Aloe vera L. was investigated.

Materials and Methods: In vitro techniques are some of the suggested methods for rapid propagation of Aloe. In this experiment, the shoot tips of mother plants were grown in a greenhouse. After surface sterilization of the explants, they were cultured on Murashige and Skoog (1962) (MS) medium containing different concentrations of kinetin and naphthaleneacetic acid (NAA). The experiment was carried out in the form of a randomized complete design with three replications.

Results: The results showed that MS media containing 1.5 mg/L kinetin along with 0.15 or 0.3 mg/L NAA produced the highest percentage of proliferated shoots. In addition, the percentage of proliferated shoots in MS medium containing 2.0 or 2.5 mg/L benzylaminopurine (BAP) + 0.15 mg/L NAA was significantly higher than the other treatments.

Conclusions: Analysis of the interactive effects of NAA, kinetin and BAP on shoot proliferation showed that most of the proliferated shoots produced in MS medium containing 1.0 mg/L BAP + 1.0 mg/L kinetin + 0.15 mg/L NAA were significantly different from other treatments. Rooting quality was greater in MS media containing 1.0 mg/L IBA than a 1.0 mg/L NAA treatment.

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