Apoptosis-Like Cell Death in Leishmania Major Treated with HESA A, an Herbal Marine Compound

AUTHORS

Jasem Saki ORCID 1 , Khalil saki 1 , Reza Arjmand ORCID 2 , *

1 Alimentary Tract Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Parasitology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

How to Cite: Saki J, saki K , Arjmand R. Apoptosis-Like Cell Death in Leishmania Major Treated with HESA A, an Herbal Marine Compound, Jundishapur J Nat Pharm Prod.2021 In Press(In Press): e99060. doi: 10.5812/jjnpp.99060.

ARTICLE INFORMATION

Jundishapur Journal of Natural Pharmaceutical Products: In Press (In Press); e99060
Published Online: May 30, 2021
Article Type: Research Article
Received: October 23, 2019
Revised: December 26, 2020
Accepted: January 12, 2021
Primary Published scheduled for 16 (3)
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Abstract

Background & Objectives: The first drug for leishmaniasis treatment is pentavalent antimony compounds, which indicated great side effects. This study was aimed to assess apoptosis induction by HESA A, an herbal marine compound in L. major promastigotes.

Methods: Leishmania major promastigotes were treated with HESA-A in different increasing concentrations ranged from 1.625-120 µg/ml, and amphotericin B and the phenomenon of Apoptosis in the parasite was evaluated by MTT, flow cytometry, and DNA fragmentation tests.

Results: The IC50 value of the compound and amphotericin B at 72 h was estimated at 2.81µg/ml and 40 µg/ml, respectively. After 72 h of the adjacency of L. major promastigotes with IC50 dose (2.81 µg/ml), the percentage of promastigotes in early and late apoptosis phases in the treated group was 5.4% and 60.4%, respectively. DNA fragmentation of L. major promastigotes treated with 2.81 µg/ml for 72 h was observed.

Conclusion: HESA-A with significant induction of Apoptosis in L.major promastigotes, it can be a plausible hope in the treatment of cutaneous leishmaniasis.

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© 2021, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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