Development and Evaluation of a Real-Time TaqMan-PCR for the Detection of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients


H Ghaffari 1 , * , O Obeidi 2 , M Dehghan 1 , B Chahardouli 1 , K Alimoghaddam 1 , A Gharebaghian 2 , AR Shamshiri 1 , A Ghavamzadeh 1

1 Assistant Professor, Hematology, Oncology and Bone Marrow Transplantation Research Center; Tehran University of Medical Sciences, Tehran, Iran

2 Research Assistant, Iran Blood Transfusion Organization Research Center, Tehran, Iran

How to Cite: Ghaffari H, Obeidi O, Dehghan M, Chahardouli B, Alimoghaddam K, et al. Development and Evaluation of a Real-Time TaqMan-PCR for the Detection of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients, Shiraz E-Med J. 2006 ; 7(3):e93680.


Shiraz E-Medical Journal: 7 (3); e93680
Published Online: July 01, 2006
Article Type: Research Article
Received: May 14, 2019
Accepted: July 01, 2006


Introduction: Cytomegalovirus (CMV) has been recognized as the most important viral pathogen in persons undergoing
bone marrow transplantation (BMT). In this study, we present the development of a TaqMan-based real-time PCR
assay to quantify human cytomegalovirus (CMV) DNA in peripheral blood leukocytes (PBLs) of bone marrow transplantation

Materials and Methods: A plasmid containing the target sequence from the pp65 region (UL83) of CMV was constructed
as a positive control template. Serial dilutions of 107 to 101 plasmids per assay were prepared. Peripheral blood
samples were collected from patients after transplantation. CMV DNA was quantified by RQ-PCR in parallel with the
pp65 antigenemia assay in PBL samples.

Results: The real-time PCR assay could detect CMV DNA in patient's samples with a wide linear range, from 10 to over
107 copies of CMV. Real-time PCR assay results correlated with those of the CMV pp65 antigenemia assay (P < 0.0001).

Discussion: The TaqMan assay may be a useful tool for rapid quantification of CMV infection and for monitoring of
CMV reactivation in bone marrow transplantation recipients. The results of both quantitative assays were significantly
correlated; however, the RQ-PCR assay was more sensitive than the pp65 antigenemia assay.



  • 1.

    The references are available in the PDF file.

  • © 2006, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.