The anti-apoptotic effects of atorvastatin in isoproterenol induced experimental heart failure

AUTHORS

Yousef Doustar 1 , * , Daryoush Mohajeri 1

1 Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

How to Cite: Doustar Y, Mohajeri D. The anti-apoptotic effects of atorvastatin in isoproterenol induced experimental heart failure, Zahedan J Res Med Sci. 2011 ; 13(2):e94060.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 13 (2); e94060
Published Online: July 20, 2010
Article Type: Research Article
Received: May 31, 2010
Accepted: June 22, 2010
READ FULL TEXT

Abstract

Background: Heart failure is one of the most important factors of death in industrialized countries. This study was performed to assess the anti-apoptotic effects of atorvastatin in isoproterenol induced experimental heart failure in rats.

Materials and Method: Fifty male Wistar rats were randomly divided into 5 groups of 10 animals each, including: 1-healthy control, 2-patient (heart failure) control, 3- patient treated with low dose of atorvastatin, 4- patient treated with moderate dose of atorvastatin, and 5- patient treated with high dose of atorvastatin. For inducing heart failure, isoproterenol was injected subcutaneously at a dose of 0.5 mg/kg daily for 10 days. Groups 1&2 received only normal saline (10 ml/kg). Groups 3-5 received atorvastatin at 5, 10 and 15 mg/kg respectively. All treatments were administered orally in 10 ml/kg normal saline solution, three weeks before isoproterenol injection and continued until the end of trial.  Twenty-four hours after the last treatment, the rats were killed and histological sections from left ventricles were prepared through Tunnel staining method. Apoptotic cells were counted by the light microscope. Data were analyzed statistically by one-way ANOVA analysis.

Results: In group 2, isoproterenol treatment increased significantly in the numbers of apoptotic cells in comparison with healthy group (p<0.001). In groups 3-5 atorvastatin (5, 10 and 15 mg/kg) caused significant decrease (p<0.05, p<0.01 and p<0.001, respectively) in the numbers of apoptotic cells in comparison with group 2.

Conclusion: The results showed that atorvastatin may prevent isoproterenol induced cardiomyocytes apoptotosis in the rats.

Fulltext

The full text of this article is available on the PDF file.

References

  • 1.

    The References of this article are available on the PDF file.

  • © 2011, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
    COMMENTS

    LEAVE A COMMENT HERE: