Hepatoprotective effect of ethanolic extract of Crocus sativus L. (Saffron) stigma in comparison with silymarin against rifampin induced hepatotoxicity in rats

AUTHORS

Daryoush Mohajeri 1 , * , Yousef Doustar 2 , Ali Rezaei 3 , Mehran Mesgari-Abbasi 4

1 Associate Professor of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

2 Assistant Professor of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

3 Associate Professor of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

4 Assistant Professor of Veterinary, Drug Resistant Research Center, Tabriz Medical University and Health Services, , Tabriz, Iran.

How to Cite: Mohajeri D, Doustar Y, Rezaei A, Mesgari-Abbasi M. Hepatoprotective effect of ethanolic extract of Crocus sativus L. (Saffron) stigma in comparison with silymarin against rifampin induced hepatotoxicity in rats, Zahedan J Res Med Sci. 2011 ; 12(5):e94102.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 12 (5); e94102
Published Online: November 19, 2010
Article Type: Research Article
Received: June 21, 2010
Accepted: September 16, 2010
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Abstract

  Background : Anti-tuberculous drug Rifampin is a potent hepatotoxicant. The aim of the present study was to evaluate the protective effect of ethanolic extract of Crocus sativus L. stigma (EECSL.S) in comparison with standard drug silimarin against rifampin-induced hepatotoxicity in the rats.

  Materials and Method : 40 male Wistar rats with the mean body weight of 200 ± 20 gr and age of 10 weeks were randomly assigned into 5 groups of 8 animals and kept in specific cages with 12/12 h light/dark cycle at 21 ± 2 ο C . Group I as normal control received normal saline (10 ml/kg) and group II as toxicant control received rifampin (500 mg/kg). Group Ш as positive control received silymarin plus rifampin (500 mg/kg) and groups IV and V (50 mg/kg) received EECSL.S at 40 mg/kg and 80 mg/kg plus rifampin, respectively. All the treatments were carried out through the gavage dissolving in 10 ml/kg normal saline daily for 1 month. At the end of experiment, levels of liver function marker enzymes (Aspartate aminotransferase, Alanine aminotransferase and Alkaline Phosphatase), total bilirubin, albumin and total proteins were assessed in serum of the rats. Moreover, histopathological observation was assayed at the degree of hepatic injury.

  Results : In rifampin-treated rats, silymarin and EECSL.S (40 and 80 mg/kg) significantly decreased the levels of serum biomarker of hepathic injury and total bilirubin and elevated the levels of albumin and total proteins. Histopathologically, silymarin and EECSL.S ameliorated rifampin induced hepatic injury. Histopathological changes were in agreement with biochemical findings.

  Conclusion : Results indicated that EECSL.S (80 mg/kg) equals with silymarin as standard drug , point of view hepatoprotective effects against rifampin-induced hepatotoxicity

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  • © 2011, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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