Histopathological Study of the Eeffect of Rofecoxib on Healing of Stomach Surgical Wounds in the Rats

AUTHORS

Daryoush Mohajeri 1 , * , Ghafour Mousavi 2 , Ali Rezaei 3 , Hossein Jodeiri 2

1 Associated Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

2 Assistant Professor of Veterinary Surgery, Dept. of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch,Tabriz, Iran.

3 Associated Professor of Veterinary Surgery, Dept. of Clinical Science, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

How to Cite: Mohajeri D, Mousavi G, Rezaei A, Jodeiri H. Histopathological Study of the Eeffect of Rofecoxib on Healing of Stomach Surgical Wounds in the Rats, Zahedan J Res Med Sci. 2010 ; 12(1):e94348.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 12 (1); e94348
Published Online: January 19, 2010
Article Type: Research Article
Received: October 18, 2009
Accepted: December 12, 2009
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Abstract

Background: Nowadays faster healing of surgical wounds is more challenging to researchers. The aim of the present study was to histopathological evaluate of the effect of rofecoxib, a non-steroidal anti-inflammatory drug, on healing of surgical wound in rats stomach. Materials and Methods: Sixty male Wistar rats were randomly assigned into three groups (control, positive control and experimental) of twenty animals each. A gastrotomy incision with the length of 1 cm was made in greater curvature of stomach in the rats, thereafter, sutured in 2 layers. Experimental group received rofecoxib (15 mg/kg) dissolved in Dimethyl Sulfoxide (DMSO) 5% (10 mL/Kg) daily for 15 days through the gavage. Positive control and control groups received DMSO 5% (10 mL/Kg) and normal saline (10 mL/Kg), respectively, in the same manner. Histopathological studies and comparison of wound healing among the groups were carried out considering more effective agents on wound healing including proliferation of fibroblasts, angiogenesis, re-epithelialization and collagen organization in healing tissue. Significant differences among the groups were determined by one-way analysis of variance followed by Bonferroni post-test. Statistical significance was considered at p<0.05.

Results: The numbers of fibroblasts, capillary buds and organized collagen content in healing site of experimental group were significantly more than positive control and control group (p<0.001). The size of epithelial gap in experimental group was significantly lesser than two other groups (p<0.001). There were not any significant statistical differences between the control and positive control groups from the viewpoint of the above parameters.

 Conclusion: The results showed that rofecoxib possesses a beneficial effect on healing of stomach surgical wounds in rats

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