Detection of the CCR5-d32 Mutation in Patients Infected with Occult Hepatitis B

AUTHORS

Mohammad Kazemi Arababadi 1 , * , Ali Akbar Pourfathollah 2 , Abdollah Jafarzadeh 3 , Gholamhossein Hasanshahi 4 , Mohammad Reza Afrooz 5 , Mahmood Hadadian 5

1 Assistant Prof of Immunology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran

2 Prof, Dept of Immunology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran

3 Associate Prof of Immunology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran

4 Assistant Prof of Hematology, Cellular and Molecular Research Center, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran

5 BSC in Laboratory Sciences, Rafsanjan Blood Transfusion Organization, Rafsanjan, Iran

How to Cite: Kazemi Arababadi M, Pourfathollah A A, Jafarzadeh A, Hasanshahi G, Afrooz M R, et al. Detection of the CCR5-d32 Mutation in Patients Infected with Occult Hepatitis B, Zahedan J Res Med Sci. 2009 ; 11(2):e94401.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 11 (2); e94401
Published Online: August 20, 2009
Article Type: Research Article
Received: September 21, 2008
Accepted: May 09, 2009
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Abstract

Background: Occult hepatitis B infection (OBI) is a form of viral hepatitis in which the HBV-DNA is present in blood while the HBsAg is undetectable. As differences in genetics and immunological responses have important role on OBI we were persuaded to analyze the known CCR5-D32 mutation (a chemokine receptor) in patients with OBI. Materials and Methods: In this descriptive study, 3700 blood samples that were HBsAg negative were collected during March 2006 to February 2007, from Rafsanjan blood transfusion services. All samples were tested for anti-HBc antibody. The HBsAg negative and anti-HBc positive samples were analyzed for HBV-DNA by PCR technique. The HBV-DNA positive samples were regarded as OBI cases. Finally, the Gap-PCR was performed to examine the d32 mutation in OBI patients. Results: The analysis of results showed that 352 (9.51%) cases of HBsAg negative samples were positive for anti-HBc. Following examination of HBsAg negative and anti-HBc positive samples for HBV-DNA by PCR based methods we found that 57(16.1%) cases had HBV-DNA. None of the OBI patients had d32 mutation in CCR5 chemokine receptor whereas 2(2%) of controls had heterozigotic form of this mutation. Conclusion: Based on the results of this study it seems that CCR5-D32 mutation does not affect the immune response against HBV to make OBI. Occult Hepatitis B Infection, CCR5, HBsAg, HBV-DNA, d32 Mutation

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  • © 2009, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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