The role of serine/threonine protein phosphatases in the inhibitory effects of low frequency stimulasion in perforant path kindled seizures acquisition

AUTHORS

mehdi sadegh 1 , * , Seyed javad Mirnajafizadeh 2

1 Instructor, Neuroscience Research Center, Kerman University of Medical Sciences and Health Services, Kerman, Iran and Dept of Physiology, Faculty of Medicine, Zabol University of Medical Sciences and Health Services, Zabol, Iran

2 Associate Prof, Neuroscience Research Center, Kerman University of Medical Sciences and Health Services, Kerman, Iran and Dept of Physiology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran

How to Cite: sadegh M, Mirnajafizadeh S J. The role of serine/threonine protein phosphatases in the inhibitory effects of low frequency stimulasion in perforant path kindled seizures acquisition, Zahedan J Res Med Sci. 2009 ; 11(1):e94429.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 11 (1); e94429
Published Online: March 11, 2009
Article Type: Research Article
Received: June 21, 2008
Accepted: February 28, 2009
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Abstract

Background: The use of low-frequency electrical stimulation (LFS) as a therapy for epilepsy is
currently being studied in experimental animals and patients with epilepsy. In the present study, we
investigated the role of serine/threonine protein phosphatases in the inhibitory effects of LFS on
perforant path kindling acquisition.
Materials and Methods: Sixty four male Wistar rats were stimulated by perforant path
stimulation in a rapid kindling manner (6 stimulations per day). The LFS (1 Hz) was applied
immediately after termination of each kindling stimulation. The FK506 (1μM; i.c.v.), a
serine/threonine protein phosphatase PP2B inhibitor and okadaic acid (1μM;i.c.v.), a
serine/threonine protein phosphatases PP1/2A inhibitor, were daily microinjected into the left
ventricle 10 min before starting the stimulation protocol. A two-way ANOVA was done to compare
the seizure parameters of different groups. The effect of LFS on behavioral seizure scores was
analyzed using the nonparametric Kruskal Wallis and Mann Whitney U tests. P value less than
0.05 was considered as the level of significance.
Results: Appling LFS immediately after kindling stimulation significantly retarded the kindling
acquisition and delayed the expression of different kindled seizure stages. In addition, LFS
significantly reduced the increment of daily after-discharge duration during kindling development.
Microinjection of neither FK506 nor okadaic acid had significant effect on the antiepileptogenic
effect of LFS on kindling parameters.
Conclusion: Our findings showed that activation of PP1/2A and PP2B, which play a critical
role in LFS, induced down-regulation of synaptic strength, had no role in mediating the inhibitory
effects of LFS on perforant path kindled seizures.

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  • © 2009, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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