Evaluation of a direct PCR in comparison with routine microscopy and In vitro culture for diagnosis of cutaneous leishmaniasis.

AUTHORS

B Fouladi 1 , Iraj Sharifi 2 , Seyyed Mohammad Hashemi Shahri 3 , HR Moradgholi 4 , A Sarabandi 5 , Adel Ebrahimzadeh 3 , A Fazaeli 3 , *

1 Dept of Parasitology, Faculty of Medicine, Zabol University of Medical Sciences and Health Services Zabol, Iran.

2 Kerman Research Center for Leishmaniasis, Kerman University of Medical Sciences and Health Services Kerman, Iran

3 Zahedan Research Center for Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences and Health Services Zahedan, Iran.

4 Zahedan Center for Health Services

5 Zahedan Islamic Azad University

How to Cite: Fouladi B, Sharifi I, Hashemi Shahri S M , Moradgholi H, Sarabandi A, et al. Evaluation of a direct PCR in comparison with routine microscopy and In vitro culture for diagnosis of cutaneous leishmaniasis., Zahedan J Res Med Sci. 2007 ; 9(3):e94788.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 9 (3); e94788
Published Online: August 25, 2007
Article Type: Research Article
Received: June 17, 2007
Accepted: July 26, 2007
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Abstract

Background: Cutaneous leishmainasis (CL) is highly prevalent in several provinces of Iran,
having increased during the recent decades. The diagnosis of CL in most of clinical laboratories is
usually performed using routine microscopy. However, this method is not sensitive enough, and the
assessment and utilization of other methods, including a variety of PCR techniques, have been taken
to consideration. In the present study, a direct PCR, based on kDNA primers, in comparison with
the microscopic examination and in vitro NNN culture was evaluated for the detection of CL.
Materials & Methods: The scrapings were taken from 73 patients from Mirjaveh, Sistan &
Baluchestan province, and subjected to the comparative diagnoses.
Results: The results showed that 38.4%, 55.5% and 63.2% of the specimens were positive by
microscopy, PCR and NNN culture, respectively. The parasite species were also characterized by
the current PCR. Separate comparisons of both microscopy and PCR methods with NNN culture,
showed that the sensitivity of the PCR (76%) is higher than that of microscopy (61%). The
calculated specificity, however, was 100% for microscopy and 73% for PCR.
Conclusion: In addition to the higher sensitivity, this particular PCR, which uses species-specific
primers, has a major advantage of identification of Leishmania species at the same time. It is,
therefore, concluded that this PCR technique can be a suitable complement to the routine
microscopic examination for diagnosis and identification of the parasite species from suspected
leishmaniasis.

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  • © 2007, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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