Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines

AUTHORS

M hashemi 1 , * , S Ghavami 1

1 Clinical Biochemistry Dept, Faculty of Medicine, Zahedan University of Medical Sciences and health services, Zahedan, Iran.

How to Cite: hashemi M, Ghavami S. Effects of estradiol, progesterone and testosterone on proliferation of human breast cancer cell lines, Zahedan J Res Med Sci. 2005 ; 7(1):e94974.

ARTICLE INFORMATION

Zahedan Journal of Research in Medical Sciences: 7 (1); e94974
Published Online: March 26, 2005
Article Type: Research Article
Received: February 11, 2004
Accepted: March 24, 2005
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Abstract

Background: Steroid hormones affect the proliferation of breast cancer. The aim of this study
was to test the effect of estradiol, progesterone and testosterone on MCF-7 and MDA-MB468
human breast cancer cell line.
Methods and Materials: The human breast cancer cell line MCF-7 and MDA-MB468 were grown
in charcoal striped serum in the presence and absence of 10 nM 17- β-estradiol with different
concentration of progesterone and testosterone for 72 hrs. Growth of cells evaluated by MTT
method.
Results: The results demonstrated that estradiol produced an increase in the cell proliferation
rate of MCF-7 cells grown in the presence of charcoal-stripped serum, but had no effect on MDAMB468
cells. While progesterone had no effect on cell growth when tested alone on both cell lines,
it significantly inhibited the growth of MCF-7 cells in the presence of 10 nM estradiol. Testosterone
had no effect on cell growth of MDA-MB468 cells in the presence or absence of estradiol. In MCF-
7 cells, testosterone significantly inhibited the growth of these cells in the absence or presence of
estradiol.
Conclusions: Proliferation of breast cancer cells are hormone dependent or hormone
independent.

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  • © 2005, Zahedan Journal of Research in Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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